Probing Mechanoregulation of Neuronal Differentiation by Plasma Lithography Patterned Elastomeric Substrates

CORC > 自动化研究所 > 中国科学院自动化研究所 > 复杂系统管理与控制国家重点实验室 > 先进控制与自动化团队

	Probing Mechanoregulation of Neuronal Differentiation by Plasma Lithography Patterned Elastomeric Substrates
	Nam, Ki-Hwan 1,2; Jamilpour, Nima 1; Mfoumou, Etienne 1; Wang, Fei-Yue3 ; Zhang, Donna D.4; Wong, Pak Kin 1
刊名	SCIENTIFIC REPORTS
	2014-11-07
卷号	4
英文摘要	Cells sense and interpret mechanical cues, including cell-cell and cell-substrate interactions, in the microenvironment to collectively regulate various physiological functions. Understanding the influences of these mechanical factors on cell behavior is critical for fundamental cell biology and for the development of novel strategies in regenerative medicine. Here, we demonstrate plasma lithography patterning on elastomeric substrates for elucidating the influences of mechanical cues on neuronal differentiation and neuritogenesis. The neuroblastoma cells form neuronal spheres on plasma-treated regions, which geometrically confine the cells over two weeks. The elastic modulus of the elastomer is controlled simultaneously by the crosslinker concentration. The cell-substrate mechanical interactions are also investigated by controlling the size of neuronal spheres with different cell seeding densities. These physical cues are shown to modulate with the formation of focal adhesions, neurite outgrowth, and the morphology of neuroblastoma. By systematic adjustment of these cues, along with computational biomechanical analysis, we demonstrate the interrelated mechanoregulatory effects of substrate elasticity and cell size. Taken together, our results reveal that the neuronal differentiation and neuritogenesis of neuroblastoma cells are collectively regulated via the cell-substrate mechanical interactions.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	ACID-INDUCED DIFFERENTIATION ; COLLECTIVE CELL-MIGRATION ; COLLOIDAL QUANTUM DOTS ; HUMAN NEUROBLASTOMA ; STEM-CELLS ; MECHANICAL-PROPERTIES ; GENE-EXPRESSION ; RIGIDITY ; STIFFNESS ; STRESS
收录类别	SCI
语种	英语
WOS记录号	WOS:000344385100017
内容类型	期刊论文
源URL	[http://ir.ia.ac.cn/handle/173211/3674]
专题	自动化研究所_复杂系统管理与控制国家重点实验室_先进控制与自动化团队
作者单位	1.Univ Arizona, Dept Aerosp & Mech Engn, Tucson, AZ 85721 USA 2.Korea Basic Sci Inst, Ctr Analyt Instrumentat Dev, Taejon 305806, South Korea 3.Chinese Acad Sci, Inst Automat, Key Lab Complex Syst & Intelligence Sci, Beijing, Peoples R China 4.Univ Arizona, Dept Pharmacol & Toxicol, Tucson, AZ 85721 USA
推荐引用方式 GB/T 7714	Nam, Ki-Hwan,Jamilpour, Nima,Mfoumou, Etienne,et al. Probing Mechanoregulation of Neuronal Differentiation by Plasma Lithography Patterned Elastomeric Substrates[J]. SCIENTIFIC REPORTS,2014,4.
APA	Nam, Ki-Hwan,Jamilpour, Nima,Mfoumou, Etienne,Wang, Fei-Yue,Zhang, Donna D.,&Wong, Pak Kin.(2014).Probing Mechanoregulation of Neuronal Differentiation by Plasma Lithography Patterned Elastomeric Substrates.SCIENTIFIC REPORTS,4.
MLA	Nam, Ki-Hwan,et al."Probing Mechanoregulation of Neuronal Differentiation by Plasma Lithography Patterned Elastomeric Substrates".SCIENTIFIC REPORTS 4(2014).