Apoplastic sugar may be lost from grape berries and retrieved in pedicels

doi:10.1093/plphys/kiac262

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	Apoplastic sugar may be lost from grape berries and retrieved in pedicels
	Zhang, Yun 5; Chang, Ben-Min ; Burdet, Berenice 2; Dai, Zhanwu 3,4; Delrot, Serge 4; Keller, Markus
刊名	PLANT PHYSIOLOGY
	2022
卷号	190 期号:1 页码:592-604
ISSN号	0032-0889
DOI	10.1093/plphys/kiac262
文献子类	Article
英文摘要	In ripening grape (Vitis sp.) berries, the combination of rapid sugar import, apoplastic phloem unloading, and water discharge via the xylem creates a potential risk for apoplastic sugar to be lost from the berries. We investigated the likelihood of such sugar loss and a possible sugar retrieval mechanism in the pedicels of different Vitis genotypes. Infusion of D-glucose-1-C-13 or L-glucose-1-C-13 to the stylar end of attached berries demonstrated that both sugars can be leached from the berries, but only the nontransport sugar L-glucose moved beyond the pedicels. No C-13 enrichment was found in peduncles and leaves. Genes encoding 10 sugar transporters were expressed in the pedicels throughout grape ripening. Using an immunofluorescence technique, we localized the sucrose transporter SUC27 to pedicel xylem parenchyma cells. These results indicate that pedicels possess the molecular machinery for sugar retrieval from the apoplast. Plasmodesmata were observed between vascular parenchyma cells in pedicels, and movement of the symplastically mobile dye carboxyfluorescein demonstrated that the symplastic connection is physiologically functional. Taken together, the chemical, molecular, and anatomical evidence gathered here supports the idea that some apoplastic sugar can be leached from grape berries and is effectively retrieved in a two-step process in the pedicels. First, sugar transporters may actively retrieve leached sugar from the xylem. Second, retrieved sugar may move symplastically to the pedicel parenchyma for local use or storage, or to the phloem for recycling back to the berry.
学科主题	Plant Sciences
电子版国际标准刊号	1532-2548
出版地	CARY
WOS关键词	VITIS-VINIFERA ; SOLUTE ACCUMULATION ; XYLEM PARENCHYMA ; PHLOEM ; TRANSPORT ; SUCROSE ; WATER ; FRUIT ; LEAF ; PATHWAY
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	OXFORD UNIV PRESS INC
WOS记录号	WOS:000808228300001
资助机构	Washington State University's College of Agricultural, Human, and Natural Resource Sciences, Department of Horticulture, and Viticulture and Enology Program ; Institut National de la Recherche Agronomique ; University of Bordeaux ; Chateau Ste. Michelle Distinguished Professorship in Viticulture
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28636]
专题	中科院北方资源植物重点实验室
作者单位	1.Belgrano 471, RA-5561 Mendoza, Argentina 2.Agr & Agri Food Canada, Summerland Res & Dev Ctr, Summerland, BC V0H 1Z0, Canada 3.Univ Bordeaux, ISVV, INRAE, Villenave Dornon, France 4.Ste Michelle Wine Estates, Prosser, WA USA 5.Washington State Univ, Irrigated Agr Res & Extens Ctr, Dept Hort, Prosser, WA 99350 USA 6.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yun,Chang, Ben-Min,Burdet, Berenice,et al. Apoplastic sugar may be lost from grape berries and retrieved in pedicels[J]. PLANT PHYSIOLOGY,2022,190(1):592-604.
APA	Zhang, Yun,Chang, Ben-Min,Burdet, Berenice,Dai, Zhanwu,Delrot, Serge,&Keller, Markus.(2022).Apoplastic sugar may be lost from grape berries and retrieved in pedicels.PLANT PHYSIOLOGY,190(1),592-604.
MLA	Zhang, Yun,et al."Apoplastic sugar may be lost from grape berries and retrieved in pedicels".PLANT PHYSIOLOGY 190.1(2022):592-604.