Extraction kinetic model of polysaccharide from Codonopsis pilosula and the application of polysaccharide in wound healing

doi:10.1088/1748-605X/ac5008

	Extraction kinetic model of polysaccharide from Codonopsis pilosula and the application of polysaccharide in wound healing
	Wang, Chenliang; Zhang, Yuchun; Xue, Hongyan; Yang, Mingjun; Leng, Feifan; Wang, Yonggang
刊名	BIOMEDICAL MATERIALS
	2022-03-01
卷号	17 期号:2
关键词	Codonopsis pilosula polysaccharide extraction kinetic model microcapsules wound repair
ISSN号	1748-6041
DOI	10.1088/1748-605X/ac5008
英文摘要	The crude polysaccharide (CPNP) of Codonopsis pilosula was obtained by hot-water extraction technology. The extraction kinetic model established according to Fick's first law of diffusion and related parameters of polysaccharide was studied. CPNP microcapsules were prepared by blending with sodium alginate, Ca2+ ions and crude CPNP. The quality control (drug loading rate, embedding rate and release rate, etc) of CPNP microcapsules were analyzed by pharmacopeas standards. The structure feature of CPNP microcapsules also were determined with various methods. The wound healing ability of CPNP microcapsules loading with different concentration of CPNP was evaluated using the rat wound model. The activity of various enzymes and the expression levels of pro-inflammatory factors in the model skin tissue also were determined by enzyme linked immunosorbent assay (ELISA). Hematoxylin-eosin staining (HE), Masson, immunohistochemistry were used to investigate the external application effect of CPNP microcapsules on skin wound repair. The extraction kinetics of CPNP was established with the linear correlation coefficient (R-2) of 0.83-0.93, implied that the extraction process was fitted well with the Fick's first law of diffusion. The CPNP has good compatibility with sodium alginate and Ca2+ ions by SEM and TEM observation, and the particle size of CPNP microcapsules was 21.25 +/- 2.84 mu m with the good degradation rate, loading rate (61.59%) and encapsulation rate (55.99%), maximum swelling rate (397.380 +/- 25.321%). Compared with control group, the redness, and swelling, bleeding, infection, and exudate of the damaged skin decreased significantly after CPNP microcapsules treatment, and the CPNP microcapsules groups exhibited good wound healing function with less inflammatory cell infiltration. The pathological structure showed that in the CPNP microcapsules group, more newborn capillaries, complete skin structure, and relatively tight and orderly arrangement of collagen fibers were observed in the skin of rats. CPNP microcapsules could effectively inhibit the high expression of pro-inflammatory factors in damaged skin, and significantly increase the contents of related enzymes (GSH-Px, T-AOC, LPO) and collagen fibers. The relative expression levels of genes (VEGF and miRNA21) in the CPNP microcapsules group were higher than those in the model group and the negative group. The above results suggested that the CPNP microcapsules could controlled-release the CPNP to the wound surface, and then played a better role in antibacterial, anti-inflammatory and skin wound repair.
WOS研究方向	Engineering ; Materials Science
语种	英语
出版者	IOP Publishing Ltd
WOS记录号	WOS:000754819600001
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/154778]
专题	生命科学与工程学院经济管理学院
作者单位	Lanzhou Univ Technol, Sch Life Sci & Engn, Langongping Rd 287, Lanzhou 730050, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Wang, Chenliang,Zhang, Yuchun,Xue, Hongyan,et al. Extraction kinetic model of polysaccharide from Codonopsis pilosula and the application of polysaccharide in wound healing[J]. BIOMEDICAL MATERIALS,2022,17(2).
APA	Wang, Chenliang,Zhang, Yuchun,Xue, Hongyan,Yang, Mingjun,Leng, Feifan,&Wang, Yonggang.(2022).Extraction kinetic model of polysaccharide from Codonopsis pilosula and the application of polysaccharide in wound healing.BIOMEDICAL MATERIALS,17(2).
MLA	Wang, Chenliang,et al."Extraction kinetic model of polysaccharide from Codonopsis pilosula and the application of polysaccharide in wound healing".BIOMEDICAL MATERIALS 17.2(2022).