Bubble-based microrobots enable digital assembly of heterogeneous microtissue modules
Ge ZX(葛治星)1,3,4; Dai LG(代利国)1,3,4; Zhao, Junhua1,5,6; Yu HB(于海波)1,4; Yang WG(杨文广)2; Liao X(寮欣)1,3,4; Tan WJ(谭文君)1,3,4; Jiao ND(焦念东)1,4; Wang ZN(王振宁)5,6; Liu LQ(刘连庆)1,4
刊名BIOFABRICATION
2022
卷号14期号:2页码:1-14
关键词bubble microrobots digital micromirror device (DMD) high-throughput fabrication reconfigurable assembly
ISSN号1758-5082
产权排序1
英文摘要

The specific spatial distribution of tissue generates a heterogeneous micromechanical environment that provides ideal conditions for diverse functions such as regeneration and angiogenesis. However, to manufacture microscale multicellular heterogeneous tissue modules in vitro and then assemble them into specific functional units is still a challenging task. In this study, a novel method for the digital assembly of heterogeneous microtissue modules is proposed. This technique utilizes the flexibility of digital micromirror device-based optical projection lithography and the manipulability of bubble-based microrobots in a liquid environment. The results indicate that multicellular microstructures can be fabricated by increasing the inlets of the microfluidic chip. Upon altering the exposure time, the Young's modulus of the entire module and different regions of each module can be fine-tuned to mimic normal tissue. The surface morphology, mechanical properties, and internal structure of the constructed bionic peritoneum were similar to those of the real peritoneum. Overall, this work demonstrates the potential of this system to produce and control the posture of modules and simulate peritoneal metastasis using reconfigurable manipulation.

资助项目National Key R&D Program of China[2018YFB1304900] ; National Natural Science Foundation of China[61925307] ; National Natural Science Foundation of China[61727811and 61973298] ; CAS Interdisciplinary Innovation Team[JCTD-2019-09] ; CAS Interdisciplinary Innovation Team[QYZDB-SSW-JSC008] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[Y201943] ; Joint Project in Key Area of Liaoning Province Natural Science Foundation[2019-KF-01-10]
WOS关键词CONTINUOUS-FLOW LITHOGRAPHY ; LIQUID INTERFACE PRODUCTION ; 3-DIMENSIONAL CELL-CULTURE ; FABRICATION ; STEREOLITHOGRAPHY ; MICROSTRUCTURES ; CONSTRUCTS ; MICROBEADS
WOS研究方向Engineering ; Materials Science
语种英语
WOS记录号WOS:000775507100001
资助机构National Key R&D Program of China [2018YFB1304900] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [61925307, 61727811and 61973298] ; CAS Interdisciplinary Innovation Team [JCTD-2019-09, QYZDB-SSW-JSC008] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences [Y201943] ; Joint Project in Key Area of Liaoning Province Natural Science Foundation [2019-KF-01-10]
内容类型期刊论文
源URL[http://ir.sia.cn/handle/173321/30745]  
专题沈阳自动化研究所_机器人学研究室
通讯作者Yu HB(于海波); Wang ZN(王振宁); Liu LQ(刘连庆)
作者单位1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
2.School of Electromechanical and Automotive Engineering, Yantai University, Yantai 264005, China
3.University of Chinese Academy of Sciences, Beijing 100049, China
4.Institutes for Robotics and Intelligent Manufacturing, Chinese Academy of Sciences, Shenyang 110016, China
5.Department of Surgical Oncology and General Surgery, The First Hospital of China Medical University, Shenyang 110016, China
6.Key Laboratory of Precision Diagnosis and Treatment of Gastrointestinal Tumors, Ministry of Education, Shenyang 110016, China
推荐引用方式
GB/T 7714
Ge ZX,Dai LG,Zhao, Junhua,et al. Bubble-based microrobots enable digital assembly of heterogeneous microtissue modules[J]. BIOFABRICATION,2022,14(2):1-14.
APA Ge ZX.,Dai LG.,Zhao, Junhua.,Yu HB.,Yang WG.,...&Liu LQ.(2022).Bubble-based microrobots enable digital assembly of heterogeneous microtissue modules.BIOFABRICATION,14(2),1-14.
MLA Ge ZX,et al."Bubble-based microrobots enable digital assembly of heterogeneous microtissue modules".BIOFABRICATION 14.2(2022):1-14.
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