RNA-silencing nanoprobes for effective activation and dynamic imaging of neural stem cell differentiation

doi:10.7150/thno.35032

	RNA-silencing nanoprobes for effective activation and dynamic imaging of neural stem cell differentiation
	Zhang, Ruili 2; Wang, Zhe 4; Yang, Zuo 2; Wang, Linlin 2; Wang, Zhongdi 2; Chen, Baihang 2; Wang, Zhongliang 2; Tian, Jie1,2,3
刊名	THERANOSTICS
	2019
卷号	9 期号:18 页码:5386-5395
关键词	gold nanoparticle gene silencing imaging drug delivery neural stem cell
ISSN号	1838-7640
DOI	10.7150/thno.35032
通讯作者	Wang, Zhongliang(wangzl@xidian.edu.cn) ; Tian, Jie(tian@ieee.org)
英文摘要	To achieve the clinical potential of neural stem cells (NSCs), it is crucial to activate NSC differentiation into neurons and simultaneously monitor the process of NSC differentiation. However, there are many challenges associated with regulating and tracking NSC differentiation. Methods: We developed a redox-responsive multifunctional nanocomplex with a disulfide bond-cvNC-for the delivery of siRNAs to induce NSC differentiation through sequence-specific RNA interference (RNAi) and real-time imaging of sequential mRNA expression during differentiation. The stability and specificity of cvNCs were studied in vitro. Controlled release of siRNA, gene silencing efficiency, as well as real-time imaging of cvNCs on Tubb3 and Fox3 mRNAs during NSC differentiation were evaluated. Results: The introduction of a redox-sensitive disulfide bond not only ensures the remarkable performance of cvNC, such as high stability, controlled siRNA release, and enhanced gene silencing efficiency, but also effectively stimulates NSC differentiation into neurons. More importantly, the cvNC can track NSC differentiation in real-time by monitoring the sequential expression of mRNAs. Conclusion: Our study indicates that cvNC can serve as a robust system for exploring NSCs differentiation process as well as other biological events in living cells.
资助项目	National Key Research and Development Program of China[2017YFC1309100] ; National Key Research and Development Program of China[2017YFA0205200] ; National Natural Science Foundation of China[81671753] ; National Natural Science Foundation of China[81227901] ; National Natural Science Foundation of China[21804104] ; National Natural Science Foundation of China[81501524] ; National 1000 Young Talents Program of China ; Natural Science Basic Research Plan in Shaanxi Province of China[2019JQ-139] ; Natural Science Basic Research Plan in Shaanxi Province of China[2018JM2041] ; Fundamental Research Funds for the Central Universities[JB191211] ; Fundamental Research Funds for the Central Universities[JB191207] ; Fundamental Research Funds for the Central Universities[JB191208]
WOS关键词	SIRNA DELIVERY ; GOLD NANOPARTICLES ; CANCER ; RESISTANCE ; PROGRESS ; DISEASE ; ACID
WOS研究方向	Research & Experimental Medicine
语种	英语
出版者	IVYSPRING INT PUBL
WOS记录号	WOS:000474899500018
资助机构	National Key Research and Development Program of China ; National Natural Science Foundation of China ; National 1000 Young Talents Program of China ; Natural Science Basic Research Plan in Shaanxi Province of China ; Fundamental Research Funds for the Central Universities
内容类型	期刊论文
源URL	[http://ir.ia.ac.cn/handle/173211/26873]
专题	中国科学院自动化研究所
通讯作者	Wang, Zhongliang; Tian, Jie
作者单位	1.Beihang Univ, Sch Med, Beijing Adv Innovat Ctr Big Data Based Precis Med, Beijing 100191, Peoples R China 2.Xidian Univ, Sch Life Sci & Technol, Minist Educ, Engn Res Ctr Mol & Neuroimaging, Xian 710126, Shaanxi, Peoples R China 3.Chinese Acad Sci, Inst Automat, Key Lab Mol Imaging, Beijing 100190, Peoples R China 4.Natl Inst Biomed Imaging & Bioengn, Lab Mol Imaging & Nanomed, NIH, Bethesda, MD 20892 USA
推荐引用方式 GB/T 7714	Zhang, Ruili,Wang, Zhe,Yang, Zuo,et al. RNA-silencing nanoprobes for effective activation and dynamic imaging of neural stem cell differentiation[J]. THERANOSTICS,2019,9(18):5386-5395.
APA	Zhang, Ruili.,Wang, Zhe.,Yang, Zuo.,Wang, Linlin.,Wang, Zhongdi.,...&Tian, Jie.(2019).RNA-silencing nanoprobes for effective activation and dynamic imaging of neural stem cell differentiation.THERANOSTICS,9(18),5386-5395.
MLA	Zhang, Ruili,et al."RNA-silencing nanoprobes for effective activation and dynamic imaging of neural stem cell differentiation".THERANOSTICS 9.18(2019):5386-5395.