Heterogeneous transportation of alpha(1B)-adrenoceptor in living cells | |
Guan, Yinghua ; Xu, Ming ; Liang, Zhangyi ; Xu, Ning ; Lu, Zhizhen ; Han, Qide ; Zhang, Youyi ; Zhao, Xin Sheng | |
刊名 | 生物物理化学 |
2007 | |
关键词 | adrenoceptor single particle tracking living cell GREEN FLUORESCENT PROTEIN HAND-OVER-HAND ALPHA(1)-ADRENOCEPTOR SUBTYPES CELLULAR-LOCALIZATION SUBCELLULAR-LOCALIZATION MEDIATED INTERNALIZATION RECEPTOR SUBTYPES LIGAND-BINDING EXPRESSION INFECTION |
DOI | 10.1016/j.bpc.2007.01.009 |
英文摘要 | The heterogeneous motion of alpha(1B)-adrenoceptor (alpha(1B)-AR) was visualized in living cells with BODIPY-labeled antagonist of AR by single molecule fluorescence microscopy at high spatial resolution. The moving trajectory was reconstructed by precise localization (better than 20 nm) with a least-square fit of a two-dimensional Gaussian point spread function to each single spot. Trajectory analysis revealed two apparent groups of movements: directed motion and hindered motion. The directed motion had speeds higher than 0.1 mu m/s. The histogram of diffusion coefficients of the hindered motion showed distinction between the cell membrane and the cytoplasm: the diffusion coefficient was lower near the cell membrane than in the internal cytoplasm, suggesting that alpha(1B)-AR was located or trapped in different networks, which was consistent with the natural distribution of cytoskeleton in living cells. These results suggested that the heterogeneity in the motion of alpha(1B)-AR in living cell might be associated with different localizations of cell skeleton proteins in the cell, which could provide molecular insight of AR regulation in living cells. (c) 2007 Elsevier B.V. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000245829700003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Biophysics; Chemistry, Physical; SCI(E); 8; ARTICLE; 3; 149-154; 127 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/250984] |
专题 | 化学与分子工程学院 |
推荐引用方式 GB/T 7714 | Guan, Yinghua,Xu, Ming,Liang, Zhangyi,et al. Heterogeneous transportation of alpha(1B)-adrenoceptor in living cells[J]. 生物物理化学,2007. |
APA | Guan, Yinghua.,Xu, Ming.,Liang, Zhangyi.,Xu, Ning.,Lu, Zhizhen.,...&Zhao, Xin Sheng.(2007).Heterogeneous transportation of alpha(1B)-adrenoceptor in living cells.生物物理化学. |
MLA | Guan, Yinghua,et al."Heterogeneous transportation of alpha(1B)-adrenoceptor in living cells".生物物理化学 (2007). |
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