Optical molecular imaging is a new in-vivo and real-time imaging technology, which can truly reflect the expression of a gene or the biological characteristics of a macromolecular, and dynamically record and display molecular events and their dynamic processes. Molecular imaging technology can, on the one hand, precisely locate the embedded tumor, realize the complete resection of the tumor and reduce the unnecessary damage to normal tissues and organs; On the other hand, it can greatly shorten the time of drug development, screening and pre-clinical research. However, the existing molecular imaging system is relatively complex and difficult to operate, which needs further improvement in practical performance and design. So we developed a novel wearable fluorescence surgical navigation system and conducted a preclinical trial of pulmonary segmentectomy in swine.