Multiscale imaging of colitis in mice using confocal laser endomicroscopy, light-sheet fluorescence microscopy, and magnetic resonance imaging

doi:10.1117/1.JBO.24.1.016003

	Multiscale imaging of colitis in mice using confocal laser endomicroscopy, light-sheet fluorescence microscopy, and magnetic resonance imaging
	Li, Tianmeng 1,2,3; Hui, Hui2,3,4 ; Hu, Chaoen2,3 ; Ma, He 1; Yang, Xin2,3 ; Tian, Jie2,3,4
刊名	JOURNAL OF BIOMEDICAL OPTICS
	2019
卷号	24 期号:1 页码:8
关键词	multimodality imaging confocal laser endomicroscopy light-sheet fluorescence microscopy magnetic resonance imaging inflammatory bowel disease
ISSN号	1083-3668
DOI	10.1117/1.JBO.24.1.016003
通讯作者	Ma, He(mahe@bmie.neu.edu.cn) ; Yang, Xin(xin.yang@ia.ac.cn) ; Tian, Jie(jie.tian@ia.ac.cn)
英文摘要	The objective of our study is to develop a multimodality approach by combining magnetic resonance imaging (MRI) and optical imaging methods to assess acute murine colitis at the macro- and microscopic level. In vivo MRI is used to measure the cross-sectional areas of colons at the macroscopic level. Dual-color confocal laser endomicroscopy (CLE) allows in vivo examination of the fluorescently labeled epithelial cells and micro-vessels in the mucosa with a spatial resolution of similar to 1.4 mu m during ongoing endoscopy. To further validate the structural changes of the colons in three-dimensions, ex vivo light-sheet fluorescence microscopy (LSFM) is applied for in-toto imaging of cleared colon sections. MRI, LSFM, and CLE findings are significantly correlated with histological scoring (p < 0.01) and the inflammation-associated activity index (p < 0.01). Our multimodality imaging technique permits visualization of mucosa in colitis at different scales, which can enhance our understanding of the pathogenesis of inflammatory bowel diseases. (C) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License.
资助项目	National Key Research and Development Program of China[2016YFC0103803] ; National Key Research and Development Program of China[2017YFA0700401] ; National Key Research and Development Program of China[2017YFA0205200] ; National Natural Science Foundation of China[81527805] ; National Natural Science Foundation of China[81671851] ; National Natural Science Foundation of China[81227901] ; Strategic Priority Research Program[XDBS01030200] ; Scientific Instrument RD Programs[GJJSTD20170004] ; Scientific Instrument RD Programs[YJKYYQ20170075] ; Frontier Science Key Research Program from the Chinese Academy of Sciences[QYZDJ-SSW-JSC005] ; Beijing Municipal Science and Technology Commission[Z161100002616022]
WOS关键词	INFLAMMATORY-BOWEL-DISEASE ; MOUSE MODEL ; COLONOSCOPY ; MUCOSA ; CANCER
WOS研究方向	Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
语种	英语
出版者	SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
WOS记录号	WOS:000463884000008
资助机构	National Key Research and Development Program of China ; National Natural Science Foundation of China ; Strategic Priority Research Program ; Scientific Instrument RD Programs ; Frontier Science Key Research Program from the Chinese Academy of Sciences ; Beijing Municipal Science and Technology Commission
内容类型	期刊论文
源URL	[http://ir.ia.ac.cn/handle/173211/24928]
专题	中国科学院自动化研究所
通讯作者	Ma, He; Yang, Xin; Tian, Jie
作者单位	1.Northeastern Univ, Sino Dutch Biomed & Informat Engn Sch, Shenyang, Liaoning, Peoples R China 2.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing, Peoples R China 3.Inst Automat, Beijing Key Lab Mol Imaging, Beijing, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Li, Tianmeng,Hui, Hui,Hu, Chaoen,et al. Multiscale imaging of colitis in mice using confocal laser endomicroscopy, light-sheet fluorescence microscopy, and magnetic resonance imaging[J]. JOURNAL OF BIOMEDICAL OPTICS,2019,24(1):8.
APA	Li, Tianmeng,Hui, Hui,Hu, Chaoen,Ma, He,Yang, Xin,&Tian, Jie.(2019).Multiscale imaging of colitis in mice using confocal laser endomicroscopy, light-sheet fluorescence microscopy, and magnetic resonance imaging.JOURNAL OF BIOMEDICAL OPTICS,24(1),8.
MLA	Li, Tianmeng,et al."Multiscale imaging of colitis in mice using confocal laser endomicroscopy, light-sheet fluorescence microscopy, and magnetic resonance imaging".JOURNAL OF BIOMEDICAL OPTICS 24.1(2019):8.