A novel plectin/integrin-targeted bispecific molecular probe for magnetic resonance/near-infrared imaging of pancreatic cancer

CORC > 自动化研究所 > 中国科学院自动化研究所 > 中国科学院分子影像重点实验室

	A novel plectin/integrin-targeted bispecific molecular probe for magnetic resonance/near-infrared imaging of pancreatic cancer
	Wang Qian 1,2,3; Yan Hao 2,3,4; Jin YS(靳玉慎)2 ; Wang Zihua 4; Huang Wenhui 2; Qiu Jia 2; Kang Feiyu 3; Wang Kun2 ; Zhao Xinming 1; Tian Jie2
刊名	Biomaterials
	2018-08-26
期号	183 页码:173-184
关键词	Bispecific Peptides Early Diagnosis Molecular Imaging Pancreatic Cancer Theranostics
英文摘要	Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest human malignancies with poor patient outcomes often resulting from delayed diagnosis. Therefore, early diagnosis can lead to a better prognosis and improved outcomes. In this study, we have developed a novel conjugate complex of plectin/integrin-targeted bispecific molecular probe, termed Gd-Cy7-PTP/RGD, to be used for magnetic resonance/near-infrared imaging (MRI/NIRF) of pancreatic cancer in vivo. This bispecific molecular probe comprises four parts: Gd(III) for MRI, cyanine 7 (Cy7) for NIRF, the peptide PTP for binding to plectin-1 specifically overexpressed on the surface of PDAC cells, and the peptide RGD for targeting integrin widely expressed on pancreatic duct epithelial cells and angiogenesis. Remarkably, the combination of PTP and RGD greatly increased the targeting efficiency in vitro and in vivocompared to that of either single peptide. Moreover, such bispecific molecular probes target pancreatic neoplasms and angiogenesis simultaneously, producing a “multi-level” targeting effect confirmed by immunofluorescence testing in vitro and in vivo. Under the guidance of MRI/NIRF dual-modality imaging, NIRF-guided delineation of surgical margins during operations was successfully achieved in orthotopic pancreatic cancer. This study promotes further exploration of bispecific molecular probes for clinical application.
内容类型	期刊论文
源URL	[http://ir.ia.ac.cn/handle/173211/21695]
专题	自动化研究所_中国科学院分子影像重点实验室
作者单位	1.Department of Imaging Diagnosis National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China 2.CAS Key Laboratory of Molecular Imaging, Institute of Automation Chinese Academy of Sciences, Beijing, 100190, China 3.School of Materials Science and Engineering, Graduate School at Shenzhen, Tsinghua University, Beijing, 100190, China 4.Key Laboratory of Colloid Interface and Chemical Thermodynamics, Institute of Chemistry Chinese Academy of Sciences, Beijing, 100190, China
推荐引用方式 GB/T 7714	Wang Qian,Yan Hao,Jin YS,et al. A novel plectin/integrin-targeted bispecific molecular probe for magnetic resonance/near-infrared imaging of pancreatic cancer[J]. Biomaterials,2018(183):173-184.
APA	Wang Qian.,Yan Hao.,Jin YS.,Wang Zihua.,Huang Wenhui.,...&Tian Jie.(2018).A novel plectin/integrin-targeted bispecific molecular probe for magnetic resonance/near-infrared imaging of pancreatic cancer.Biomaterials(183),173-184.
MLA	Wang Qian,et al."A novel plectin/integrin-targeted bispecific molecular probe for magnetic resonance/near-infrared imaging of pancreatic cancer".Biomaterials .183(2018):173-184.