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浏览/检索结果: 共5条，第1-5条 帮助 已选(0)清除 条数/页：5101520253035404550556065707580859095100   排序方式：请选择作者升序作者降序题名升序题名降序发表日期升序发表日期降序提交时间升序提交时间降序 Application of next-generation sequencing technology to precision medicine in cancer: joint consensus of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology 期刊论文 CANCER BIOLOGY & MEDICINE, 2019, 卷号: 16, 期号: 1 作者:  Wu, Chunyan;  Shao, Zhimin;  Xu, Binghe;  Zhang, Qingyuan;  Xu, Ruihua 收藏  |  浏览/下载：123/0  |  提交时间：2019/12/05 Next-generation sequencing technology  cancer  consensus   Application of next-generation sequencing technology to precision medicine in cancer: joint consensus of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology 期刊论文 2019, 卷号: 16, 期号: 1, 页码: 189-204 作者:  Zhang Xuchao;  Liang Zhiyong;  Wang Shengyue;  Lu Shun;  Song Yong 收藏  |  浏览/下载：25/0  |  提交时间：2020/01/03 Next-generation sequencing technology  cancer  consensus   Characterization of the complete mitochondrial genome and phylogenetic relationship of Caranx tille (Perciformes: Carangidae) 期刊论文 MITOCHONDRIAL DNA PART A, 2016, 卷号: 27, 期号: 6, 页码: 4704-4705 作者:  Ma, Hongyu[1];  Ma, Chunyan[2];  Zhang, Heng[3];  Zhang, Xun[4];  Feng, Chunlei[5] 收藏  |  浏览/下载：4/0  |  提交时间：2020/01/02 Caranx tille  genome structure  mitochondrial genome  next-generation sequencing technology  phylogeny   DEVELOPMENT AND CHARACTERIZATION OF EST-SSR MARKERS IN OSTRYOPSIS (BETULACEAE) 期刊论文 APPLICATIONS IN PLANT SCIENCES, 2014, 卷号: 2, 期号: 2, 页码: - 作者:  Liu, BB;  Tian, B;  Ma, H;  Lu, ZQ;  Qiu, Q 收藏  |  浏览/下载：4/0  |  提交时间：2015/12/14 Betulaceae  EST-SSR marker  next-generation sequencing technology  ortholog genes  Ostryopsis   Single-molecule emulsion PCR in microfluidic droplets 期刊论文 2012 Zhu, Zhi; Jenkins, Gareth; Zhang, Wenhua; Zhang, Mingxia; Guan, Zhichao; Yang, Chaoyong James; 杨朝勇 收藏  |  浏览/下载：3/0  |  提交时间：2013/03/26 Single-molecule amplification  Emulsion PCR  Microfluidic droplets  High-throughput screening  Next generation DNA sequencing  Rare mutation detection  The application of microfluidic droplet PCR for single-molecule amplification and analysis has recently been extensively studied. Microfluidic droplet technology has the advantages of compartmentalizing reactions into discrete volumes, performing highly parallel reactions in monodisperse droplets, reducing cross-contamination between droplets, eliminating PCR bias and nonspecific amplification, as well as enabling fast amplification with rapid thermocycling. Here, we have reviewed the important technical breakthroughs of microfluidic droplet PCR in the past five years and their applications to single-molecule amplification and analysis, such as high-throughput screening, next generation DNA sequencing, and quantitative detection of rare mutations. Although the utilization of microfluidic droplet single-molecule PCR is still in the early stages, its great potential has already been demonstrated and will provide novel solutions to today's biomedical engineering challenges in single-molecule amplification and analysis.