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Reagentless near-infrared determination of cholesterol in undiluted human serum using interval partial least square (EI CONFERENCE) 会议论文
2011 International Conference on Smart Materials and Nanotechnology in Engineering, SMNE 2011, September 17, 2011 - September 18, 2011, Wuhan, China
Gao H.; Lu Q.; Huang F.
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In order to determination of cholesterol in human serum with no reagent using nearinfrared (NIR) spectroscopy. Interval partial least square (iPLS) was proposed as an effective variable selection approach for multivariate calibration. For this purpose  an independent sample set was employed to evaluate the prediction ability of the resulting model. The spectrum was split into different interval. Then  the informative region of cholesterol (1688-1760nm)  in which the PLS model has a low RMSEP with 0.241mmol/L and a high R with 0.975  is selected with 23 intervals. The results indicate that  the informative region of cholesterol can be obtained by iPLS and applied to design the simpler reagentless NIR instruments for inexpensive cholesterol measurement in future. (2012) Trans Tech Publications  Switzerland.  


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