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Enhanced activity and stability of Rhizomucor miehei lipase by mutating N-linked glycosylation site and its application in biodiesel production
期刊论文
FUEL, 2021, 卷号: 304, 页码: 11
作者:
Tian, Miao
;
Fu, Junying
;
Wang, Zhiyuan
;
Miao, Changlin
;
Lv, Pengmei
收藏
  |  
浏览/下载:61/0
  |  
提交时间:2021/10/27
Rhizomucor miehei lipase
N-linked glycosylation site
Thermostability
Methanol tolerance
Biodiesel
Visualizing Soluble Protein Mutants by Using Monomeric Red Fluorescent Protein as a Reporter for Directed Evolution
期刊论文
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2018, 卷号: 185, 期号: 1, 页码: 81-90
作者:
Yang, Xiaobing
;
Zhao, Zongbao K.
;
Wang, Xueying
;
Wang, Lei
;
Lin, Xinping
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  |  
浏览/下载:28/0
  |  
提交时间:2019/06/20
Directed Evolution
Fusion Protein
Library Screening
Monomeric Red Fluorescent Protein
Soluble Protein Reporter
Directed evolution of mevalonate kinase in Escherichia coli by random mutagenesis for improved lycopene
期刊论文
RSC ADVANCES, 2018, 卷号: 8, 期号: 27, 页码: 15021-15028
作者:
Chen, Hailin
;
Liu, Changqing
;
Li, Meijie
;
Zhang, Haibo
;
Xian, Mo
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  |  
浏览/下载:30/0
  |  
提交时间:2018/12/21
Improvement of alkalophilicity of an alkaline xylanase Xyn11A-LC from Bacillus sp SN5 by random mutation and Glu135 saturation mutagenesis
期刊论文
BMC BIOTECHNOLOGY, 2016, 卷号: 16
作者:
Bai, Wenqin
;
Cao, Yufan
;
Liu, Jun
;
Wang, Qinhong
;
Jia, Zhenhu
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  |  
浏览/下载:31/0
  |  
提交时间:2017/03/13
Xylanase
Alkalophilicity
Error-prone PCR
Site-directed mutation
Site-saturation mutagenesis
Catalytic efficiency
Semi-rational engineering of cytochrome CYP153A from Marinobacter aquaeolei for improved omega-hydroxylation activity towards oleic acid
期刊论文
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2016, 卷号: 100, 期号: 20, 页码: 8779-8788
作者:
Duan, Yan
;
Ba, Lina
;
Gao, Jianwei
;
Gao, Xianxing
;
Zhu, Dunming
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  |  
浏览/下载:43/0
  |  
提交时间:2017/03/21
Cytochrome CYP153A
Oleic acid
Electron transfer
Site-directed saturation mutagenesis
Iterative saturation mutagenesis
Identification and thermoadaptation engineering of thermostability conferring residue of deep sea bacterial alpha-amylase AMY121
期刊论文
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2016, 卷号: 126, 页码: 56-63
作者:
Yang, Jian
;
Li, Lizhen
;
Xiao, Yunzhu
;
Li, Jie
;
Long, Lijuan
收藏
  |  
浏览/下载:16/0
  |  
提交时间:2016/12/26
Deep sea bacterial amylase
Thermostability
Hotspot residue
Semi-rational design
Semi-Rational Engineering a Carbonyl Reductase for the Enantioselective Reduction of beta-Amino Ketones
期刊论文
ACS CATALYSIS, 2015, 卷号: 5, 期号: 4, 页码: 2452-2457
作者:
Zhang, Dalong
;
Chen, Xi
;
Chi, Jing
;
Feng, Jinhui
;
Wu, Qiaqing
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  |  
浏览/下载:27/0
  |  
提交时间:2016/06/23
chiral gamma-amino alcohols
beta-amino ketones
enzyme engineering
carbonyl reductase
enantioselective reduction
Substrate channel evolution of an esterase for the synthesis of cilastatin
期刊论文
Catal. Sci. Technol., 2015, 卷号: 5, 期号: 5, 页码: 2622-2629
作者:
Luan ZJ(栾正娇)
;
Li FL(李福龙)
;
Dou S(窦帅)
;
Chen Q(陈琦)
;
Zhou JH(周佳海)
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  |  
浏览/下载:65/0
  |  
提交时间:2016/12/05
Reshaping an Enzyme Binding Pocket for Enhanced and Inverted Stereoselectivity: Use of Smallest Amino Acid Alphabets in Directed Evolution
期刊论文
Angew. Chem.-Int. Edit., 2015, 卷号: 54, 期号: 42, 页码: 12410-12415
作者:
Sun ZT(孙周通)
;
理查德·郎斯达勒
;
Kong XD(孔旭东)
;
Xu JH(许建和)
;
Zhou JH(周佳海)
收藏
  |  
浏览/下载:36/0
  |  
提交时间:2016/12/05
Site-saturation mutagenesis of central tyrosine 195 leading to diverse product specificities of an alpha-cyclodextrin glycosyltransferase from Paenibacillus sp 602-1
期刊论文
JOURNAL OF BIOTECHNOLOGY, 2014, 卷号: 170, 页码: 10-16
作者:
Xie, Ting
;
Song, Binghong
;
Yue, Yang
;
Chao, Yapeng
;
Qian, Shijun
收藏
  |  
浏览/下载:4/0
  |  
提交时间:2020/01/06
Cyclodextrin glycosyltransferases
Central site Tyr195
Cyclodextrin
Product specificity
Site-directed saturation mutagenesis
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