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科研机构
山东大学 [65]
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期刊论文 [61]
会议论文 [4]
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2019 [2]
2018 [3]
2017 [5]
2016 [8]
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专题:山东大学
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T5 exonuclease-dependent assembly offers a low-cost method for efficient cloning and site-directed mutagenesis
期刊论文
NUCLEIC ACIDS RESEARCH, 2019, 卷号: 47, 期号: 3
作者:
Xia, Yongzhen
;
Li, Kai
;
Li, Jingjing
;
Wang, Tianqi
;
Gu, Lichuan
收藏
  |  
浏览/下载:18/0
  |  
提交时间:2019/12/11
The phage T4 DNA ligase in vivo improves the survival-coupled bacterial mutagenesis
期刊论文
MICROBIAL CELL FACTORIES, 2019, 卷号: 18, 期号: 1
作者:
Wang, Junshu
;
Liu, Fapeng
;
Su, Tianyuan
;
Chang, Yizhao
;
Guo, Qi
收藏
  |  
浏览/下载:15/0
  |  
提交时间:2019/12/11
T4 DNA ligase
Microbial mutagenesis
Atmospheric and room temperature
plasma (ARTP)
Polyhydroxybutyrate (PHB)
Rational designed mutagenesis of levansucrase from Bacillus licheniformis 8-37-0-1 for product specificity study
期刊论文
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2018, 卷号: 102, 期号: 7, 页码: 3217-3228
作者:
He, Chunjuan
;
Yang, Yirui
;
Zhao, Renfei
;
Qu, Jingyao
;
Jin, Lan
收藏
  |  
浏览/下载:4/0
  |  
提交时间:2019/12/11
Levansucrase
Transfructosylation
Levan
Site-directed mutagenesis
Fructo-oligosaccharides
Enhancement in catalytic activity of Aspergillus niger XynB by selective site-directed mutagenesis of active site amino acids
期刊论文
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2018, 卷号: 102, 期号: 1, 页码: 249-260
作者:
Wu, Xiuyun
;
Tian, Zhennan
;
Jiang, Xukai
;
Zhang, Qun
;
Wang, Lushan
收藏
  |  
浏览/下载:5/0
  |  
提交时间:2019/12/11
GH11xylanase
Active site architecture
Rational design
Binding
affinity
Catalytic activity
Structural and biochemical characterization of the plant type III polyketide synthases of the liverwort Marchantia paleacea
期刊论文
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2018, 卷号: 125, 页码: 95-105
作者:
Yu, Hai-Na
;
Liu, Xin-Yan
;
Gao, Shuai
;
Sun, Bin
;
Zheng, Hong-Bo
收藏
  |  
浏览/下载:9/0
  |  
提交时间:2019/12/11
Chalcone synthase
Type III polyketide synthase
Claisen condensation
Lactonization
Crystal structure
Mutagenesis
Revised mechanism and improved efficiency of the quikchange site-directed mutagenesis method
期刊论文
Methods in Molecular Biology, 2017, 卷号: 1498, 页码: 367-374
作者:
Xia, Yongzhen
;
Xun, Luying
收藏
  |  
浏览/下载:2/0
  |  
提交时间:2019/12/16
Overlap PCR
PCR
QuikChange
Site-directed mutagenesis
Development of a counterselectable seamless mutagenesis system in lactic acid bacteria
期刊论文
MICROBIAL CELL FACTORIES, 2017, 卷号: 16
作者:
Xin, Yongping
;
Guo, Tingting
;
Mu, Yingli
;
Kong, Jian
收藏
  |  
浏览/下载:9/0
  |  
提交时间:2019/12/12
Lactic acid bacteria
Temperature-sensitive plasmid
Seamless
mutagenesis
Counterselectable marker
pheS
Mutagenesis and immunological evaluation of group A streptococcal C5a peptidase as an antigen for vaccine development and as a carrier protein for glycoconjugate vaccine design
期刊论文
RSC ADVANCES, 2017, 卷号: 7, 期号: 67, 页码: 42056-42063
作者:
Li, Hui
;
Wang, Subo
;
Zhao, Yisheng
;
Chen, Zonggang
;
Gu, Guofeng
收藏
  |  
浏览/下载:9/0
  |  
提交时间:2019/12/12
Identification of Important Amino Acids in Gal2p for Improving the L-arabinose Transport and Metabolism in Saccharomyces cerevisiae
期刊论文
FRONTIERS IN MICROBIOLOGY, 2017, 卷号: 8
作者:
Wang, Chengqiang
;
Li, Yanwei
;
Qiu, Chenxi
;
Wang, Shihao
;
Ma, Jinjin
收藏
  |  
浏览/下载:5/0
  |  
提交时间:2019/12/12
L-arabinose transport
Gal2p
site-directed mutagenesis
key residue
metabolism
budding yeast
Improvement of clavulanic acid production in Streptomyces clavuligerus F613-1 by using a claR-neo reporter strategy
期刊论文
ELECTRONIC JOURNAL OF BIOTECHNOLOGY, 2017, 卷号: 28, 页码: 41-46
作者:
Qin, Ronghuo
;
Zhong, Chuanqing
;
Zong, Gongli
;
Fu, Jiafang
;
Pang, Xiuhua
收藏
  |  
浏览/下载:3/0
  |  
提交时间:2019/12/12
claR
Clavulanic acid biosynthesis
Co-transcription
Fermentation
Fusion
Kanamycin
Mutagenesis
Mutagens
Promoter-less
Reporter gene
Transcriptional regulator
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