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科研机构
过程工程研究所 [43]
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期刊论文 [36]
学位论文 [7]
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2021 [4]
2019 [1]
2018 [2]
2017 [6]
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Tailored nanodisc immobilization for one-step purification and reconstitution of cytochrome P450: A tool for membrane proteins' hard cases
期刊论文
JOURNAL OF SEPARATION SCIENCE, 2021, 页码: 12
作者:
Zhao, Lan
;
Tao, Jiaoli
;
Huang, Yongdong
;
Zhu, Kai
;
Du, Yuxiang
收藏
  |  
浏览/下载:60/0
  |  
提交时间:2021/08/31
cytochrome P450
membrane protein purification
nanodiscs
nanodisc-immobilized chromatography
reconstitution
Purification and characterization of the receptor-binding domain of SARS-CoV-2 spike protein from Escherichia coli
期刊论文
ENGINEERING IN LIFE SCIENCES, 2021, 页码: 8
作者:
He, Yunxia
;
Qi, Jinming
;
Xiao, Lucheng
;
Shen, Lijuan
;
Yu, Weili
收藏
  |  
浏览/下载:63/0
  |  
提交时间:2021/08/31
COVID‐
19
purification
RBD
SARS‐
CoV‐
2
spike protein
In vitro preparation of uniform and nucleic acid free hepatitis B core particles through an optimized disassembly-purification-reassembly process
期刊论文
PROTEIN EXPRESSION AND PURIFICATION, 2021, 卷号: 178, 页码: 7
作者:
Zhang, Yao
;
Liu, Yongdong
;
Zhang, Bingyang
;
Yin, Shuang
;
Li, Xiunan
收藏
  |  
浏览/下载:51/0
  |  
提交时间:2021/08/31
Hepatitis B core (HBc)
Uniform VLPs
Nucleic acid removal
Disassembly-purification-reassembly
Biochemical characterization and molecular docking of cloned xylanase gene from Bacillus subtilis RTS expressed in E. coli
期刊论文
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2021, 卷号: 168, 页码: 310-321
作者:
Saleem, Aimen
;
Waris, Saboora
;
Ahmed, Toheed
;
Tabassum, Romana
收藏
  |  
浏览/下载:10/0
  |  
提交时间:2021/03/29
Cloning
Sequencing
Genetic characterization
AutoDock Vina
Expression, Purification, and Polyethylene Glycol Site-Specific Modification of Recombinant Human Interleukin 24 in Escherichia coli
期刊论文
PROTEIN JOURNAL, 2019, 卷号: 38, 期号: 5, 页码: 576-585
作者:
Zhang, Yao
;
Ma, Qunfeng
;
Wang, Junfeng
;
Ge, Jianlin
;
Hua, Jilei
收藏
  |  
浏览/下载:104/0
  |  
提交时间:2019/11/12
IL-24
Gene engineering proteins
PEG site-specific modification
Half-life
Strong hydrophobicity enables efficient purification of HBc VLPs displaying various antigen epitopes through hydrophobic interaction chromatography
期刊论文
BIOCHEMICAL ENGINEERING JOURNAL, 2018, 卷号: 140, 页码: 157-167
作者:
Li, Zhengjun
;
Wei, Jiangxue
;
Yang, Yanli
;
Ma, Xiaowei
;
Hou, Baidong
收藏
  |  
浏览/下载:112/0
  |  
提交时间:2018/12/28
Hbc-vlp
Hydrophobic Interaction Chromatography
Surface Hydrophobicity Properties
Rational Design
Vaccine Candidates
基于乙肝核心抗原病毒样颗粒及其衍生物颗粒性质的纯化和应用过程设计
学位论文
: 中国科学院大学, 2018
作者:
李正军
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  |  
浏览/下载:16/0
  |  
提交时间:2020/06/17
乙肝核心抗原病毒样颗粒,疏水色谱,颗粒学,加热处理,载体
Purification and characterization of a long-acting ciliary neurotrophic factor via genetically fused with an albumin-binding domain
期刊论文
PROTEIN EXPRESSION AND PURIFICATION, 2017, 卷号: 139, 期号: NOV, 页码: 14-20
作者:
Xu, Longfu
;
Zhang, Chun
;
Liu, Liping
;
Zhang, Yao
;
Wang, Qi
收藏
  |  
浏览/下载:18/0
  |  
提交时间:2017/11/27
Ciliary Neurotrophic Factor
Albumin-binding Domain
Pegylation
Circulatory Half-life
Pharmacokinetic
High hydrostatic pressure enables almost 100% refolding of recombinant human ciliary neurotrophic factor from inclusion bodies at high concentration
期刊论文
PROTEIN EXPRESSION AND PURIFICATION, 2017, 卷号: 133, 页码: 152-159
作者:
Wang, Qi
;
Liu, Yongdong
;
Zhang, Chun
;
Guo, Fangxia
;
Feng, Cui
收藏
  |  
浏览/下载:19/0
  |  
提交时间:2017/07/10
rhCNTF
High hydrostatic pressure
Inclusion bodies
Hydrophobic aggregation
Refolding
Purification and assembling a fused capsid protein as an enterovirus 71 vaccine candidate from inclusion bodies to pentamer-based nanoparticles
期刊论文
BIOCHEMICAL ENGINEERING JOURNAL, 2017, 卷号: 117, 页码: 139-146
作者:
Xue, Ling
;
Liu, Jiangning
;
Wang, Qi
;
Zhang, Chun
;
Xu, Longfu
收藏
  |  
浏览/下载:45/0
  |  
提交时间:2017/03/24
Enterovirus 71 (EV71)
Virus-like particles (VLPs)
Assembling
Fused antigen
Inclusion bodies
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