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Oriented immobilization of penicillin G acylase on the epoxydized magnetic hydroxyl particles by phenyl acetic acid 会议论文
2nd International Conference on Chemical Engineering and Advanced Materials, CEAM 2012, Guangzhou, China, July 13, 2012 - July 15, 2012
作者:  Wang, Wei*;  Li, Zhonghai;  Li, Jilie;  Wu, Yaohui
收藏  |  浏览/下载:9/0  |  提交时间:2019/12/28
Protein A for human IgG oriented immobilization on silicon surface for an imaging ellipsometry biosensor (EI CONFERENCE) 会议论文
31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009, September 2, 2009 - September 6, 2009, Minneapolis, MN, United states
Meng Y.; Chen B.
收藏  |  浏览/下载:14/0  |  提交时间:2013/03/25
Imaging ellipsometry biosensor was developed on the basement of imaging ellipsometry for thin-film and surface characterization. Using protein A to immobilize Human IgG on silicon surface for the imaging ellipsometry biosensor immunoassay was studied. As a result  The Human IgG immobilized on silicon surface by protein A bound much more polyclonal antibody than that on hydrophobic surface directly  which indicated that protein A could be used to immobilize Human IgG molecules in a highly oriented manner and Human IgG molecule could maintain its native configuration well on the silicon surface. Furthermore  the absorption quantity of Protein A affecting on interaction of Human IgG and antibody molecules was investigated. The surface concentration of Human IgG and antibody molecules was enhanced as well as the increasing of the absorption quantity of Protein A  but there are no obvious increase when the ratio of surface concentration of Protein A layer to that of saturated adsorption is larger than 80%. However  even decrease slightly. 2009 IEEE.  
Protein A for Human IgG Oriented Immobilization on Silicon Surface for an Imaging Ellipsometry biosensor 会议论文
2009
Meng Y. L.; Chen B.; Ieee
收藏  |  浏览/下载:7/0  |  提交时间:2013/03/28


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