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浏览/检索结果: 共1条，第1-1条 帮助 限定条件 内容类型：会议论文     已选(0)清除 条数/页：5101520253035404550556065707580859095100   排序方式：请选择作者升序作者降序题名升序题名降序发表日期升序发表日期降序提交时间升序提交时间降序 A study of interaction between FITC- labeled antibody and optical fiber surface (EI CONFERENCE) 会议论文 ICO20: Materials and Nanostructures, August 21, 2005 - August 26, 2005, Changchun, China Chao K. F.; Zhang Y. L.; Kong X. G.; Zeng Q. H.; Liu R. L.; Wang X.; Feng L. Y.; Sun Y. J. 收藏  |  浏览/下载：31/0  |  提交时间：2013/03/25 The immobilization of antibodies on solid surface has been widely studied in elucidating immunoassay and in developing immunosensor. In this paper  FITC-labeled goat anti-human IgG were covalently immobilized on 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde (Glu) to modify the distal end of a fiber-optic probe. The higher density and stability of FITC-labeled goat anti-human IgG could be obtained on the fiber surface modified with APTES-Glu than that on the non-modified fiber surface by evanescent excited fluorescence spectroscopy. Furthermore  red shift of the FITC fluorescence dye emission peak was observed on different fiber surface  exhibiting different interaction between FITC- labeled antibody and surface. Finally  under these conditions antigen-antibody reaction was investigated as an immunosensor. Detection limit of the evanescent wave optic-fiber biosensor is 10ng/ml.