Separation of Four Phenylpropanoid Glycosides from a Chinese Herb by HSCCC

	Separation of Four Phenylpropanoid Glycosides from a Chinese Herb by HSCCC
	Zhang, Lin 1; Yue, Hui-Lan 1,2; Zhao, Xiao-Hui 1,2,3; Li, Jing 1; Shao, Yun 1
刊名	journal of chromatographic science
	2015-07-01
卷号	53 期号:6 页码:860-865
中文摘要	four phenylpropanoid glycosides (ppgs), such as echinacoside, wiede-mannioside c, forsythoside b and verbascoside, were isolated and purified from the tibetan medicinal herb pedicularis longiflora rudolph. var. tubiformis (klotz) tsoong by high-speed counter-current chromatography (hsccc) combined with macroporous resin (mr) column separation for the first time. in the present study, the two-phase solvent system composed of ethyl acetate-n-butanol-water (10 : 6: 15, v/v/v) was used for hsccc separation. a total of 3.5 mg of echinacoside, 12.6 mg of wiede-mannioside c, 22.7 mg of forsythoside b and 48.7 mg of verbascoside with the purity of 93.6, 97.9, 97.8 and 98.1%, respectively, were obtained from 120 mg of crude sample. the hsccc fractions were analyzed by hplc, and the chemical structures were identified by h-1 nmr and c-13 nmr. the results demonstrate that mr coupled with hsccc is a powerful technique for separation of ppgs from natural products.
英文摘要	four phenylpropanoid glycosides (ppgs), such as echinacoside, wiede-mannioside c, forsythoside b and verbascoside, were isolated and purified from the tibetan medicinal herb pedicularis longiflora rudolph. var. tubiformis (klotz) tsoong by high-speed counter-current chromatography (hsccc) combined with macroporous resin (mr) column separation for the first time. in the present study, the two-phase solvent system composed of ethyl acetate-n-butanol-water (10 : 6: 15, v/v/v) was used for hsccc separation. a total of 3.5 mg of echinacoside, 12.6 mg of wiede-mannioside c, 22.7 mg of forsythoside b and 48.7 mg of verbascoside with the purity of 93.6, 97.9, 97.8 and 98.1%, respectively, were obtained from 120 mg of crude sample. the hsccc fractions were analyzed by hplc, and the chemical structures were identified by h-1 nmr and c-13 nmr. the results demonstrate that mr coupled with hsccc is a powerful technique for separation of ppgs from natural products.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	counter-current chromatography ; stachytarpheta-cayennensis ; phenylethanoid glycosides ; cistanche-deserticola ; preparative isolation ; lamiophlomis-rotata ; purification ; antioxidant ; roth
收录类别	SCI
语种	英语
WOS记录号	WOS:000361837600004
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/5537]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 3.Chinese Acad Sci, Key Lab Tibetan Med Res, Xining 810001, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Lin,Yue, Hui-Lan,Zhao, Xiao-Hui,et al. Separation of Four Phenylpropanoid Glycosides from a Chinese Herb by HSCCC[J]. journal of chromatographic science,2015,53(6):860-865.
APA	Zhang, Lin,Yue, Hui-Lan,Zhao, Xiao-Hui,Li, Jing,&Shao, Yun.(2015).Separation of Four Phenylpropanoid Glycosides from a Chinese Herb by HSCCC.journal of chromatographic science,53(6),860-865.
MLA	Zhang, Lin,et al."Separation of Four Phenylpropanoid Glycosides from a Chinese Herb by HSCCC".journal of chromatographic science 53.6(2015):860-865.