Analysis of high-molecular-weight glutenin subunits in five amphidiploids and their parental diploid species Aegilops umbellulata and Aegilops uniaristata

	Analysis of high-molecular-weight glutenin subunits in five amphidiploids and their parental diploid species Aegilops umbellulata and Aegilops uniaristata
	Dai, Shoufen 1; Zhao, Li 1; Xue, Xiaofei 1; Jia, Yanni 1; Liu, Dengcai 1,3; Pu, Zongjun 2; Zheng, Youliang 1; Yan, Zehong 1
刊名	plant genetic resources-characterization and utilization
	2015-08-01
卷号	13 期号:2 页码:186-189
关键词	Aegilops umbellulata Aegilops uniaristata amphidiploids HMW-GS synthetic hexaploid species Triticum turgidum
中文摘要	amphidiploids serve as a bridge for transferring genes from wild species into wheat. in this study, five amphidiploids with aabbuu and aabbnn genomes were produced by spontaneous chromosome doubling of unreduced triploid f-1 gametes from crosses between diploid aegilops (a. umbellulata accessions ciae 29 and pi 226500, and a. uniaristata accession pi 554419) and tetraploid triticum turgidum (ssp. durum cultivar langdon and ssp. dicoccum accessions pi 94668 and pi 349045) species. the composition of high-molecular-weight glutenin subunits (hmw-gs) in these amphidiploids and in their parental a. umbellulata and a. uniaristata species was analysed. as expected, the amphidiploids from t. turgidum ssp. dicoccum accession pi 944668 or pi 349045 and a. umbellulata accession ciae 29 or pi 226500 and a. uniaristata accession pi 554419 showed the same hmw-gs patterns as those of their aegilops parents, because hmw-gs genes were all silenced in the t. turgidum ssp. dicoccum parents. the amphidiploids from ciae 29 and langdon inherited all of the hmw-gs genes from their parents except for the uy type. using 10 and 15% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) and 10% urea/sds-page, 11 ux and ten uy types in 16 combinations were observed in 48 a. umbellulata accessions, and two nx and two ny types in two combinations were detected in six a. uniaristata accessions. these novel hmw-gs variants may provide new genetic resources for improving the quality of wheat.
英文摘要	amphidiploids serve as a bridge for transferring genes from wild species into wheat. in this study, five amphidiploids with aabbuu and aabbnn genomes were produced by spontaneous chromosome doubling of unreduced triploid f-1 gametes from crosses between diploid aegilops (a. umbellulata accessions ciae 29 and pi 226500, and a. uniaristata accession pi 554419) and tetraploid triticum turgidum (ssp. durum cultivar langdon and ssp. dicoccum accessions pi 94668 and pi 349045) species. the composition of high-molecular-weight glutenin subunits (hmw-gs) in these amphidiploids and in their parental a. umbellulata and a. uniaristata species was analysed. as expected, the amphidiploids from t. turgidum ssp. dicoccum accession pi 944668 or pi 349045 and a. umbellulata accession ciae 29 or pi 226500 and a. uniaristata accession pi 554419 showed the same hmw-gs patterns as those of their aegilops parents, because hmw-gs genes were all silenced in the t. turgidum ssp. dicoccum parents. the amphidiploids from ciae 29 and langdon inherited all of the hmw-gs genes from their parents except for the uy type. using 10 and 15% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) and 10% urea/sds-page, 11 ux and ten uy types in 16 combinations were observed in 48 a. umbellulata accessions, and two nx and two ny types in two combinations were detected in six a. uniaristata accessions. these novel hmw-gs variants may provide new genetic resources for improving the quality of wheat.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	plant sciences ; genetics & heredity
研究领域[WOS]	plant sciences ; genetics & heredity
关键词[WOS]	synthetic hexaploid wheats ; allelic variation ; tauschii ; hybridization ; quality ; set
收录类别	SCI
语种	英语
WOS记录号	WOS:000357822000014
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/5530]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Sichuan Agr Univ, Triticeae Res Inst, Chengdu 611130, Sichuan, Peoples R China 2.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China
推荐引用方式 GB/T 7714	Dai, Shoufen,Zhao, Li,Xue, Xiaofei,et al. Analysis of high-molecular-weight glutenin subunits in five amphidiploids and their parental diploid species Aegilops umbellulata and Aegilops uniaristata[J]. plant genetic resources-characterization and utilization,2015,13(2):186-189.
APA	Dai, Shoufen.,Zhao, Li.,Xue, Xiaofei.,Jia, Yanni.,Liu, Dengcai.,...&Yan, Zehong.(2015).Analysis of high-molecular-weight glutenin subunits in five amphidiploids and their parental diploid species Aegilops umbellulata and Aegilops uniaristata.plant genetic resources-characterization and utilization,13(2),186-189.
MLA	Dai, Shoufen,et al."Analysis of high-molecular-weight glutenin subunits in five amphidiploids and their parental diploid species Aegilops umbellulata and Aegilops uniaristata".plant genetic resources-characterization and utilization 13.2(2015):186-189.