Pokemon siRNA Delivery Mediated by RGD-Modified HBV Core Protein Suppressed the Growth of Hepatocellular Carcinoma

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Pokemon siRNA Delivery Mediated by RGD-Modified HBV Core Protein Suppressed the Growth of Hepatocellular Carcinoma
	Kong, Jing 1; Liu, Xiaoping 1; Jia, Jianbo 1; Wu, Jinsheng 2; Wu, Ning 3; Chen, Jun 1; Fang, Fang 1
刊名	HUMAN GENE THERAPY METHODS
	2015-10-01
卷号	26 期号:5 页码:175-180
英文摘要	Hepatocellular carcinoma (HCC) is a deadly human malignant tumor that is among the most common cancers in the world, especially in Asia. Hepatitis B virus (HBV) infection has been well established as a high risk factor for hepatic malignance. Studies have shown that Pokemon is a master oncogene for HCC growth, suggesting it as an ideal therapeutic target. However, efficient delivery system is still lacking for Pokemon targeting treatment. In this study, we used core proteins of HBV, which is modified with RGD peptides, to construct a biomimetic vector for the delivery of Pokemon siRNAs (namely, RGD-HBc-Pokemon siRNA). Quantitative PCR and Western blot assays revealed that RGD-HBc-Pokemon siRNA possessed the highest efficiency of Pokemon suppression in HCC cells. In vitro experiments further indicated that RGD-HBc-Pokemon-siRNA exerted a higher tumor suppressor activity on HCC cell lines, evidenced by reduced proliferation and attenuated invasiveness, than Pokemon-siRNA or RGD-HBc alone. Finally, animal studies demonstrated that RGD-HBc-Pokemon siRNA suppressed the growth of HCC xenografts in mice by a greater extent than Pokemon-siRNA or RGD-HBc alone. Based on the above results, Pokemon siRNA delivery mediated by RGD-modified HBV core protein was shown to be an effective strategy of HCC gene therapy.
收录类别	SCI
语种	英语
WOS记录号	WOS:000363943500003
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/82281]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Huaiyin Inst Technol, Fac Life Sci & Chem Engn, Huaian 223003, Jiangsu, Peoples R China 2.Nanjing Med Univ, Huaian Peoples Hosp 1, Dept Hepatopancreatobiliary Surg, Huaian, Jiangsu, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Kong, Jing,Liu, Xiaoping,Jia, Jianbo,et al. Pokemon siRNA Delivery Mediated by RGD-Modified HBV Core Protein Suppressed the Growth of Hepatocellular Carcinoma[J]. HUMAN GENE THERAPY METHODS,2015,26(5):175-180.
APA	Kong, Jing.,Liu, Xiaoping.,Jia, Jianbo.,Wu, Jinsheng.,Wu, Ning.,...&Fang, Fang.(2015).Pokemon siRNA Delivery Mediated by RGD-Modified HBV Core Protein Suppressed the Growth of Hepatocellular Carcinoma.HUMAN GENE THERAPY METHODS,26(5),175-180.
MLA	Kong, Jing,et al."Pokemon siRNA Delivery Mediated by RGD-Modified HBV Core Protein Suppressed the Growth of Hepatocellular Carcinoma".HUMAN GENE THERAPY METHODS 26.5(2015):175-180.