Ferritin M of Paralichthys olivaceus possesses antimicrobial and antioxidative properties

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Ferritin M of Paralichthys olivaceus possesses antimicrobial and antioxidative properties
	Wang, Jing-jing1,2 ; Sun, Li 1
刊名	FISH PHYSIOLOGY AND BIOCHEMISTRY
	2015-08-01
卷号	41 期号:4 页码:951-959
关键词	Antimicrobial Antioxidative Ferritin Iron binding Paralichthys olivaceus
英文摘要	Ferritin is an evolutionarily conserved protein that plays a vital role in maintaining iron homeostasis. In this study, we identified a ferritin M (PoFerM) from Japanese flounder (Paralichthys olivaceus) and analyzed its biological property. PoFerM is composed of 176 amino acid residues and contains the conserved ferroxidase diiron center and the ferrihydrite nucleation center typical of M ferritins. Expression of PoFerM occurred in multiple tissues and was most abundant in blood. Bacterial infection upregulated PoFerM expression in head kidney, spleen, and liver in a time-dependent manner. Recombinant PoFerM (rPoFerM) purified from Escherichia coli exhibited iron-chelating activity and inhibited bacterial growth, whereas rPoFerMM, the mutant protein that bears alanine substitution at two conserved residues of the ferroxidase center and the ferrihydrite nucleation center, failed to do so. Oxidative protection analysis showed that rPoFerM, but not rPoFerMM, was able to alleviate the deleterious effect of H2O2-induced free radicals on plasmid DNA and primary flounder cells. Together these results indicate that PoFerM is an iron chelator with antimicrobial and antioxidative properties, all which depend on the conserved ferroxidase center and the ferrihydrite nucleation site.
收录类别	SCI
语种	英语
WOS记录号	WOS:000357660700011
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/49201]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jing-jing,Sun, Li. Ferritin M of Paralichthys olivaceus possesses antimicrobial and antioxidative properties[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2015,41(4):951-959.
APA	Wang, Jing-jing,&Sun, Li.(2015).Ferritin M of Paralichthys olivaceus possesses antimicrobial and antioxidative properties.FISH PHYSIOLOGY AND BIOCHEMISTRY,41(4),951-959.
MLA	Wang, Jing-jing,et al."Ferritin M of Paralichthys olivaceus possesses antimicrobial and antioxidative properties".FISH PHYSIOLOGY AND BIOCHEMISTRY 41.4(2015):951-959.