Interaction of d(GGTATACC)(2) and EDTA in solution

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 2011年以前论文发表（包括2011年）

	Interaction of d(GGTATACC)(2) and EDTA in solution
	Yan, JL ; Mao, XA ; Shen, LF ; Zhang, YJ
刊名	CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
	1998-05-01
卷号	19 期号:5 页码:821-823
关键词	NOESY d(GGTATACC)(2) EDTA intermolecular interactions
英文摘要	When three dimensional conformations of nucleic acid are studied with NMR methods, EDTA (ethylene diamine tetra-acetic acid) or its sodium salt has to be added in the solution in order to protect the nucleic acids from contamination by some metal ions such as Mg2+. In this paper, the interaction of d(GGTATACC)(2) and EDTA has been observed in D2O solution containing 0.05 mmol/L DNA and about 5 mmol/L EDTA. Four pairs of NOE cross peaks connecting the acetic proton (delta 3.64) of EDTA with the protons G(1)8 (delta 7.81), G(1)1' (delta 5.65), G(1)3' (delta 4.13) and G(1)2' (delta 2.54) of d(GGTATACC)(2) observed, suggesting that EDTA may form complex with d(GGTATACC)(2) possibly through hydrogen bond interactions between amino protons in DNA (H2 of G(1) and H4 of C-8) and carbonyl oxygen atoms of EDTA. The interaction of d(GGTATACC)(2) and EDTA may account for NOEs between two DNA oligomers, G(1)1' (5.65) and C(8)6 (7.47), G(1)1' (5.65) and C(8)2'/C(8)2 "(2.18).
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Multidisciplinary
研究领域[WOS]	Chemistry
关键词[WOS]	AQUEOUS-SOLUTION ; DUPLEX ; DODECAMER
收录类别	SCI
语种	英语
WOS记录号	WOS:000075179300038
公开日期	2015-12-01
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/8333]
专题	武汉物理与数学研究所_2011年以前论文发表（包括2011年）
作者单位	1.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 2.Cent China Normal Univ, Ctr Anal & Testing, Wuhan 430079, Peoples R China
推荐引用方式 GB/T 7714	Yan, JL,Mao, XA,Shen, LF,et al. Interaction of d(GGTATACC)(2) and EDTA in solution[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,1998,19(5):821-823.
APA	Yan, JL,Mao, XA,Shen, LF,&Zhang, YJ.(1998).Interaction of d(GGTATACC)(2) and EDTA in solution.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,19(5),821-823.
MLA	Yan, JL,et al."Interaction of d(GGTATACC)(2) and EDTA in solution".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 19.5(1998):821-823.