Determination of binding constants between one protein and multiple carbohydrates by affinity chromatography on a microchip

	Determination of binding constants between one protein and multiple carbohydrates by affinity chromatography on a microchip
	Liu, Xiaojun 1; Wang, Hui 2; Liang, Aiye 3; Li, Yuling 1; Gai, Hongwei 1; Lin, Bingcheng 4
刊名	journal of chromatography a
	2012-12-28
卷号	1270 页码:340-343
关键词	Binding constants Carbohydrate-protein interaction Drug screening Affinity chromatography Microchip capillary electrophoresis
英文摘要	development of rapid, reliable and high throughput methods for evaluating the interactions between different carbohydrates and a same protein is critical to carbohydrate drug development. in this study, we develop a novel strategy based on an affinity chromatography for quickly determining the binding constants of different carbohydrates to a same protein. the core of our method is the inversely proportional relationship between the binding constant and a new termed parameter, critical elution concentration (cmc). cmc is defined as the lowest concentration of displacing reagent, a series of carbohydrates herein, at which the protein specifically bond to the affinity column can be eluted off as an intact peak by the carbohydrate solution in a certain time. the interactions between a series of sulfate polysaccharides and granulocyte colony-stimulating factor (g-csf) are selected as model. through a 200 mu m long heparin affinity column microfabricated inside a channel of 50 mu m width and 20 mu m height, the binding constant of each g-csf-polysaccharide binding pair can be obtained within 1 h, around one sixth of time needed by traditional capillary electrophoresis based method. (c) 2012 elsevier b.v. all rights reserved.
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	colony-stimulating factor ; surface-plasmon resonance ; capillary-electrophoresis ; microfluidic chip ; heparin ; separation ; oligosaccharides ; glycans ; sulfate ; design
收录类别	SCI
语种	英语
WOS记录号	WOS:000312572900041
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/142903]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Jiangsu Normal Univ, Sch Chem & Engn, Xuzhou 221116, Peoples R China 2.China Pharmaceut Univ Pharmaceut Co Ltd, Nanjing 210009, Jiangsu, Peoples R China 3.Charleston So Univ, Dept Phys Sci, N Charleston, SC 29406 USA 4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Liu, Xiaojun,Wang, Hui,Liang, Aiye,et al. Determination of binding constants between one protein and multiple carbohydrates by affinity chromatography on a microchip[J]. journal of chromatography a,2012,1270:340-343.
APA	Liu, Xiaojun,Wang, Hui,Liang, Aiye,Li, Yuling,Gai, Hongwei,&Lin, Bingcheng.(2012).Determination of binding constants between one protein and multiple carbohydrates by affinity chromatography on a microchip.journal of chromatography a,1270,340-343.
MLA	Liu, Xiaojun,et al."Determination of binding constants between one protein and multiple carbohydrates by affinity chromatography on a microchip".journal of chromatography a 1270(2012):340-343.