Study on membrane characteristics of alginate-chitosan microcapsule with cell growth

	Study on membrane characteristics of alginate-chitosan microcapsule with cell growth
	Yu, Weiting 2; Song, Huiyi 2; Zheng, Guoshuang 2; Liu, Xiudong 1; Zhang, Ying 2; Ma, Xiaojun 2
刊名	journal of membrane science
	2011-07-15
卷号	377 期号:1-2 页码:214-220
关键词	Alginate-chitosan microcapsule Membrane characteristics Microencapsulated cell culture Apparent membrane
英文摘要	alginate-chitosan (ac) microcapsule with polyelectrolyte complex (pec) membrane has been attractive in biotechnological area such as cell immobilization fermentation. the pec membrane has been known important to control microcapsule performance, but the knowledge was mainly obtained with empty or drug-loaded microcapsules. in this paper, the emphasis was to explore the influence of cell growth on pec membrane characteristics, that is, to study the membrane characteristics in dynamic process. ac microcapsule entrapping yeast cells (saccharomyces cerevisiae by4741) was used as model, and the pec membrane characteristics including membrane thickness, swelling behavior, permeability, and component were studied during cell culture process. it was found that both the membrane thickness and volume swelling degree of ac microcapsule increased, however, membrane permeability decreased with the growth of entrapped yeast cells. fluorescence-labeling studies suggested the concentrated alginate layer squeezed by the proliferated cells and macromolecular metabolites layer secreted by proliferated cells contributed to above increment. the former pec membrane, together with the new layers, constituted the 'apparent membrane', which increased the diffusion resistance of substances. (c) 2011 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; technology ; physical sciences
类目[WOS]	engineering, chemical ; polymer science
研究领域[WOS]	engineering ; polymer science
关键词[WOS]	oligochitosan microcapsules ; intestinal delivery ; swelling behavior ; prepared capsule ; molecular-weight ; in-vitro ; release ; microparticles ; encapsulation ; microspheres
收录类别	SCI
语种	英语
WOS记录号	WOS:000292580000022
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/142364]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Dalian Univ, Coll Environm & Chem Engn, Dalian 116622, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Yu, Weiting,Song, Huiyi,Zheng, Guoshuang,et al. Study on membrane characteristics of alginate-chitosan microcapsule with cell growth[J]. journal of membrane science,2011,377(1-2):214-220.
APA	Yu, Weiting,Song, Huiyi,Zheng, Guoshuang,Liu, Xiudong,Zhang, Ying,&Ma, Xiaojun.(2011).Study on membrane characteristics of alginate-chitosan microcapsule with cell growth.journal of membrane science,377(1-2),214-220.
MLA	Yu, Weiting,et al."Study on membrane characteristics of alginate-chitosan microcapsule with cell growth".journal of membrane science 377.1-2(2011):214-220.