Micropillar array chips toward new immunodiagnosis

	Micropillar array chips toward new immunodiagnosis
	Li, Hong-Yi 1,2; Dauriac, Virginie 2; Thibert, Valerie 2; Senechal, Helene 2; Peltre, Gabriel 2; Zhang, Xin-Xiang 1; Descroix, Stephanie 2
刊名	lab on a chip
	2010
卷号	10 期号:19 页码:2597-2604
英文摘要	in this paper, we demonstrate the possibility to use a micropillar array to perform molecular immunodiagnosis. a polydimethylsiloxane (pdms) microdevice consisting of a rectangular array of micropillars (45 mu m in height, 100 x 100 mu m square cross section) was used to replace microchannels or gels (polyacrylamide or agarose) to perform electrokinetic separation. this microarray was used to mimic highly diluted gel and to maintain electrolyte within the pillar zone by capillary effect. the electrolyte composition (glycerol and agarose content) was investigated in order to improve protein separation by isoelectric focusing (ief). the influence of glycerol on focusing time and on the different evaporative contributions was further evaluated. in order to perform an immunodiagnostic of milk allergy, different surface treatments were optimized to prevent milk allergen adsorption on pdms surface. poly(dimethylacrylamide)-co-allyl glycidyl ether (pdma-age) as well as gelatin led to a satisfactory signal to noise ratio. finally the possibility to perform protein mixture separation using this micropillar array chip followed by immunoblotting was demonstrated by using the serum from an allergic individual, confirming the great potential of this analytical platform in the field of immunodiagnosis.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, multidisciplinary ; nanoscience & nanotechnology
研究领域[WOS]	biochemistry & molecular biology ; chemistry ; science & technology - other topics
关键词[WOS]	total analysis systems ; bovine beta-lactoglobulin ; allergen-specific ige ; capillary-electrophoresis ; microfluidic systems ; carrier ampholytes ; milk allergy ; microchip ; serum ; immunoassay
收录类别	SCI
语种	英语
WOS记录号	WOS:000281614900017
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/141709]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Peking Univ, Inst Analyt Chem, Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci,Coll Chem,Minist Educ, Beijing 100871, Peoples R China 2.PECSA, UMR 7195, F-75231 Paris 05, France
推荐引用方式 GB/T 7714	Li, Hong-Yi,Dauriac, Virginie,Thibert, Valerie,et al. Micropillar array chips toward new immunodiagnosis[J]. lab on a chip,2010,10(19):2597-2604.
APA	Li, Hong-Yi.,Dauriac, Virginie.,Thibert, Valerie.,Senechal, Helene.,Peltre, Gabriel.,...&Descroix, Stephanie.(2010).Micropillar array chips toward new immunodiagnosis.lab on a chip,10(19),2597-2604.
MLA	Li, Hong-Yi,et al."Micropillar array chips toward new immunodiagnosis".lab on a chip 10.19(2010):2597-2604.