Effects of splice sites on the intron retention in histamine H-3 receptors from rats and mice | |
Ding, Wenyong1,2,3; Lin, Lin2; Ren, Feng2; Zou, Hanfa; Duan, Ziyuan1; Dai, Jianwu1 | |
刊名 | journal of genetics and genomics
![]() |
2009-08-01 | |
卷号 | 36期号:8页码:475-482 |
关键词 | histamine H-3 receptor (H3R) alternative splicing intron retention splice site minigene |
英文摘要 | in the alternative splicing, intron retention, of histamine h-3 receptors in rats and mice, the short transcript isoforms that are excised alternatively spliced introns are easily detected in a very low level in rats and arc undetectable in mice using the regular pcr protocol. the retained introns have common 5' splice site and different 3' splice sites. the detailed mechanism for the special alternative splicing remains largely unclear. in this study, we developed a minigene splicing system to recapitulate natural alternative splicing of the receptors and investigated the effects of 5' and 3' splice sites on intron retention in hela cells. mutating weak 5' and 3' splice sites of the alternatively spliced introns toward the canonical consensus sequences promoted the splicing of the corresponding introns in rat and mouse minigenes. the effect of splice site strength was context-dependent and much more significant for the 3' splice site of the longer alternative intron than for the 3' splice site of the shorter alternative intron and the common 5' splice sites; it was also more significant in the rat minigene than in the mouse minigene. mutating the 3' splice site of the longer alternative intron resulted in almost complete splicing of the intron and made the corresponding isoform to become the nearly exclusive transcript in the rat minigene. |
WOS标题词 | science & technology ; life sciences & biomedicine |
类目[WOS] | biochemistry & molecular biology ; genetics & heredity |
研究领域[WOS] | biochemistry & molecular biology ; genetics & heredity |
关键词[WOS] | overlap extension ; isoforms ; gene ; cloning ; organization ; expression ; pcr ; recognition ; microarrays ; mutagenesis |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000268980800004 |
公开日期 | 2015-11-17 |
内容类型 | 期刊论文 |
源URL | [http://159.226.238.44/handle/321008/141464] ![]() |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Mol Dev Biol, Beijing 100080, Peoples R China 2.Dalian Med Univ, Dept Biochem & Mol Biol, Dalian 116044, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China |
推荐引用方式 GB/T 7714 | Ding, Wenyong,Lin, Lin,Ren, Feng,et al. Effects of splice sites on the intron retention in histamine H-3 receptors from rats and mice[J]. journal of genetics and genomics,2009,36(8):475-482. |
APA | Ding, Wenyong,Lin, Lin,Ren, Feng,Zou, Hanfa,Duan, Ziyuan,&Dai, Jianwu.(2009).Effects of splice sites on the intron retention in histamine H-3 receptors from rats and mice.journal of genetics and genomics,36(8),475-482. |
MLA | Ding, Wenyong,et al."Effects of splice sites on the intron retention in histamine H-3 receptors from rats and mice".journal of genetics and genomics 36.8(2009):475-482. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论