BSA-imprinted synthetic receptor for reversible template recognition

	BSA-imprinted synthetic receptor for reversible template recognition
	Wang, Huafang 1; He, Yunhua 1; He, Xiwen 1; Li, Wenyou 1; Chen, Langxing 1; Zhang, Yukui 1,2
刊名	journal of separation science
	2009-06-01
卷号	32 期号:11 页码:1981-1986
关键词	Bovine serum albumin (BSA) Chitosan High performance liquid chromatography (HPLC) Molecularly imprinted polymers (MIPs)
英文摘要	a novel approach to the manufacturing of protein-responsive imprints on a home-made chitosan substrate was established together with m-aminophenylboronic acid (apba) as a functional monomer. the produced polymers were characterized using both (1) equilibrium adsorption assays and (2) high performance liquid chromatography analysis. results confirmed that the synthesized bsa-mip (molecularly imprinted polymer) has a high affinity towards its template compared to the determined control proteins. the produced bsa-mip featured largely in its good adsorption reversibility, especially in competitive binding assays, which is of great biological significance in separations. non-specific binding was reduced to almost zero in a bsa/bhb competitive binding event. an excellent hplc profile of template recognition was found for bsa-mip, even under harsh mobile phase conditions. in the present work, the adopted trapped-template-release method permits recovery of bound bsa [1]. the strategy of making an artificial protein-receptor with high adsorption affinity and reversibility is promising in on-line isolation of target protein from complicated biological environments.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	bovine serum-albumin ; 3-aminophenylboronic acid ; functional monomer ; thin-films ; surface ; polymer ; proteins
收录类别	SCI
语种	英语
WOS记录号	WOS:000266929700024
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/141452]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Wang, Huafang,He, Yunhua,He, Xiwen,et al. BSA-imprinted synthetic receptor for reversible template recognition[J]. journal of separation science,2009,32(11):1981-1986.
APA	Wang, Huafang,He, Yunhua,He, Xiwen,Li, Wenyou,Chen, Langxing,&Zhang, Yukui.(2009).BSA-imprinted synthetic receptor for reversible template recognition.journal of separation science,32(11),1981-1986.
MLA	Wang, Huafang,et al."BSA-imprinted synthetic receptor for reversible template recognition".journal of separation science 32.11(2009):1981-1986.