Assessment of antifungal effects of a novel compound from Burkholderia cepacia against Fusarium solani by fluorescent staining

	Assessment of antifungal effects of a novel compound from Burkholderia cepacia against Fusarium solani by fluorescent staining
	Li, Xin 2,3; Quan, Chun-Shan 1; Yu, Hui-Ying ; Wang, Jian-Hua 2,3; Fan, Sheng-Di 1
刊名	world journal of microbiology & biotechnology
	2009
卷号	25 期号:1 页码:151-154
关键词	Burkholeria cepacia Fusarium solani CF66I Fluorescent staining Fungicidal Fungistatic
英文摘要	a novel compound cf66i produced by burkholeria cepacia was investigated for its antifungal effects against fusarium solani by three different fluorescent dyes. dual staining with propidium iodide (pi) and fluorescein diacetate (fda) demonstrated high doses of cf66i (120.0 mu g ml(-1)) killed the fungi by acting primarily on the cell membrane. however, at fungistatic concentration (20.0 mu g ml(-1)) of this compound, microscopic observations revealed swelling hyphae with abnormal chitin deposition, as determined by calcofluor white (cfw) staining, which was indicative of the alterations in cell wall structure. in addition, inhibition of intracellular esterases activity was observed. these results led us to conclude that low doses of cf66i probably inhibited the fungal growth by interfering with the cell metabolic pathways.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biotechnology & applied microbiology
研究领域[WOS]	biotechnology & applied microbiology
关键词[WOS]	candida-albicans ; in-vitro ; susceptibility
收录类别	SCI
语种	英语
WOS记录号	WOS:000261413100020
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/141386]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Dalian Nationalities Univ, Life Sci Coll, Dalian 116600, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Xin,Quan, Chun-Shan,Yu, Hui-Ying,et al. Assessment of antifungal effects of a novel compound from Burkholderia cepacia against Fusarium solani by fluorescent staining[J]. world journal of microbiology & biotechnology,2009,25(1):151-154.
APA	Li, Xin,Quan, Chun-Shan,Yu, Hui-Ying,Wang, Jian-Hua,&Fan, Sheng-Di.(2009).Assessment of antifungal effects of a novel compound from Burkholderia cepacia against Fusarium solani by fluorescent staining.world journal of microbiology & biotechnology,25(1),151-154.
MLA	Li, Xin,et al."Assessment of antifungal effects of a novel compound from Burkholderia cepacia against Fusarium solani by fluorescent staining".world journal of microbiology & biotechnology 25.1(2009):151-154.