Fabrication of a surface imprinted hydrogel shell over silica microspheres using bovine serum albumin as a model protein template | |
Hua, Zhendong; Zhou, Shuang; Zhao, Meiping | |
刊名 | biosensors & bioelectronics |
2009-11-15 | |
卷号 | 25期号:3页码:615-622 |
关键词 | Surface imprinting Hydrogel Bovine serum albumin Silica microsphere |
英文摘要 | surface imprinting is an effective approach to improve the template transfer efficiency in applications of molecularly imprinted polymers as biosensors and separation materials. in this paper, we tried to fabricate a surface imprinted hydrogel over silica microspheres for selective recognition of bovine serum albumin by covalent immobilization of a water-soluble uv sensitive initiator onto the surface of silica beads. the polymerization was initiated by uv radiation with n-[3-(dimethylamino)propyl]methacrylamide and n-isopropylacrylamide as the functional monomer and assistant monomer, respectively, and a thin coat of stimuli-responsive hydrogel yielded over the silica gels. the surface imprinted hydrogels exhibited specific affinity toward the template protein with an association constant (k(a)) of 2.2 x 10(5) lmol(-1) and a maximum binding capacity (q(max)) of 27.3 mg g(-1) in tris-hcl buffer (ph 7.0). the rebinding and desorption kinetics of the surface imprinted hydrogels were determined and proven to be extremely fast (about 1 min compared to 3 h for the previously prepared bulk imprinted hydrogel). besides, the hydrogel-silica core-shell particles inherit both the stimuli-responsive property of the hydrogel and the good mechanical strength of the silica beads based on the on-line evaluation with high-performance liquid chromatography. the above comprehensive merits of the obtained surface imprinted hydrogel suggest the presented approach an attractive and broadly applicable way of developing biosensors and high-performance protein separation materials. (c) 2009 elsevier b.v. all rights reserved. |
WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
类目[WOS] | biophysics ; biotechnology & applied microbiology ; chemistry, analytical ; electrochemistry ; nanoscience & nanotechnology |
研究领域[WOS] | biophysics ; biotechnology & applied microbiology ; chemistry ; electrochemistry ; science & technology - other topics |
关键词[WOS] | polyacrylamide-gel beads ; miniemulsion polymerization ; polymers ; recognition ; irradiation ; initiators ; particles ; lysozyme ; cysteine ; films |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000271931900014 |
公开日期 | 2015-11-17 |
内容类型 | 期刊论文 |
源URL | [http://159.226.238.44/handle/321008/141342] |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | Peking Univ, BNLMS, Key Lab Bioorgan Chem & Mol Engn, Coll Chem & Mol Engn,Minist Educ, Beijing 100871, Peoples R China |
推荐引用方式 GB/T 7714 | Hua, Zhendong,Zhou, Shuang,Zhao, Meiping. Fabrication of a surface imprinted hydrogel shell over silica microspheres using bovine serum albumin as a model protein template[J]. biosensors & bioelectronics,2009,25(3):615-622. |
APA | Hua, Zhendong,Zhou, Shuang,&Zhao, Meiping.(2009).Fabrication of a surface imprinted hydrogel shell over silica microspheres using bovine serum albumin as a model protein template.biosensors & bioelectronics,25(3),615-622. |
MLA | Hua, Zhendong,et al."Fabrication of a surface imprinted hydrogel shell over silica microspheres using bovine serum albumin as a model protein template".biosensors & bioelectronics 25.3(2009):615-622. |
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