Direct electrochemistry and electrocatalysis of cytochrome c immobilized on gold nanoparticles-chitosan-carbon nanotubes-modified electrode

	Direct electrochemistry and electrocatalysis of cytochrome c immobilized on gold nanoparticles-chitosan-carbon nanotubes-modified electrode
	Xiang, Cuili 1,2; Zou, Yongjin 1,2; Sun, Li-Xian 1; Xu, Fen 1
刊名	talanta
	2007-11-30
卷号	74 期号:2 页码:206-211
关键词	cytochrome c gold nanoparticles multi-walled carbon nanotubes chitosan nanohybrid direct electron transfer
英文摘要	a robust and effective nanohybrid film based on gold nanoparticles (gnps)/chitosan (chit)/multi-walled carbon nanotubes (mwnts) was prepared by a layer-by-layer self-assembly technique. cytochrome c (cyt c) was successfully immobilized on the nanohybrid film modified glassy carbon (gc) electrode by cyclic voltammetry. the direct electron transfer between cyt c and the modified electrode was investigated in detail. cyt c shows a couple of quasi-reversible and well-defined cyclic voltammetry peaks with a formal potential (e-0) of -0. 16 v (versus ag/agcl) in ph 7.0 phosphate buffer solution (pbs). the cyt c/gnps/chit/mwnts modified gc electrode gives an improved electrocatalytic activity towards the reduction of hydrogen peroxide (h2o2). the sensitivity is 92.21 mu a mm(-1) cm(-2) and the calculated apparent michael is-menten constant (k-m(app)) is 0.791 mm, indicating a high-catalytic activity of cyt c. the catalysis currents increase linearly to the h2o2 concentration in a wide range of 1.5 x 10(-6) to 5. 1 x 10(-4) m with a correlation coefficient 0.999. the detection limit is 9.0 x 10(-7) m (at the ratio of signal to noise, s/n = 3). moreover, the modified electrode displays rapid response (5 s) to h2o2, and possesses good stability and reproducibility. (c) 2007 elsevier b.v all fights reserved.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	hydrogen-peroxide ; hybrid composites ; biosensor ; film ; sensor ; interface ; oxidation ; proteins ; surfaces ; behavior
收录类别	SCI
语种	英语
WOS记录号	WOS:000251629800006
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/140521]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Mat & Thermochem Lab, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xiang, Cuili,Zou, Yongjin,Sun, Li-Xian,et al. Direct electrochemistry and electrocatalysis of cytochrome c immobilized on gold nanoparticles-chitosan-carbon nanotubes-modified electrode[J]. talanta,2007,74(2):206-211.
APA	Xiang, Cuili,Zou, Yongjin,Sun, Li-Xian,&Xu, Fen.(2007).Direct electrochemistry and electrocatalysis of cytochrome c immobilized on gold nanoparticles-chitosan-carbon nanotubes-modified electrode.talanta,74(2),206-211.
MLA	Xiang, Cuili,et al."Direct electrochemistry and electrocatalysis of cytochrome c immobilized on gold nanoparticles-chitosan-carbon nanotubes-modified electrode".talanta 74.2(2007):206-211.