Comparison of spiculogenesis in in vitro ADCP-primmorph and explants culture of marine sponge Hymeniacidon perleve with 3-TMOSPU supplementation

	Comparison of spiculogenesis in in vitro ADCP-primmorph and explants culture of marine sponge Hymeniacidon perleve with 3-TMOSPU supplementation
	Zhang, Wei ; Cao, Xupeng ; Zhang, Wei
刊名	journal of biotechnology
	2007-09-01
卷号	131 期号:2 页码:s31-s31
英文摘要	this study aims to test the feasibility of introducing functional chemical groups into biogenic silica spicules by examining the effect of supplementing a silican coupler [3-( trimethoxysilyl)propyl] urea ( 3-tmospu) as silica source in the cultures of archaeocytes-dominant-cell-population (adcp) primmorphs and explants of the marine sponge hymeniacidon perleve. analysis by fourier transform infrared spectroscopy ( ft-ir) confirmed that the organic group in 3-tmospu was introduced into silica spicules. by comparing adcp-primmorph cultures when supplemented with na2sio3, 3-tmospu supplementation showed no notable effect on the primmorphs development and cell locomotion behaviors. a decline in silicatein expression quantified by real-time rt-pcr was, however, observed during spiculogenesis. the decline was slower for the 3-tmospu group whereas significantly fewer spicules were formed. when sponge papillae explants were cultured, 3-tmospu supplementation had no negative effect on sponge growth but inhibited the growth biofouling of the diatom nitzschia closterium. by monitoring the detectable si concentration, it seemed that 3-tmospu was converted by the sponge and its conversion was related to spiculogenesis. analysis of spicule dimensional changes indicated that the inhibition of spiculogenesis by 3- tmospu supplementation was less in adcp-primmorphs culture due to lower 3-tmospu/detectable si ratio in the media.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biotechnology & applied microbiology
研究领域[WOS]	biotechnology & applied microbiology
关键词[WOS]	coral-reef sponges ; suberites-domuncula ; silicatein-alpha ; halichondria-panicea ; enzymatic-synthesis ; cell-proliferation ; catalytic-activity ; low-temperature ; growth-rates ; neutral ph
收录类别	ISTP ; SCI
语种	英语
WOS记录号	WOS:000249687100048
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/140494]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalain Inst Chem Phys, Marine Bioprod Engn Grp, Dalian, Liaoning, Peoples R China 2.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Mol Bioproc & Bioprod Lab, Adelaide, SA 5001, Australia
推荐引用方式 GB/T 7714	Zhang, Wei,Cao, Xupeng,Zhang, Wei. Comparison of spiculogenesis in in vitro ADCP-primmorph and explants culture of marine sponge Hymeniacidon perleve with 3-TMOSPU supplementation[J]. journal of biotechnology,2007,131(2):s31-s31.
APA	Zhang, Wei,Cao, Xupeng,&Zhang, Wei.(2007).Comparison of spiculogenesis in in vitro ADCP-primmorph and explants culture of marine sponge Hymeniacidon perleve with 3-TMOSPU supplementation.journal of biotechnology,131(2),s31-s31.
MLA	Zhang, Wei,et al."Comparison of spiculogenesis in in vitro ADCP-primmorph and explants culture of marine sponge Hymeniacidon perleve with 3-TMOSPU supplementation".journal of biotechnology 131.2(2007):s31-s31.