Immunoassay by graphite furnace atomic absorption spectrometry using a metal chelate as a label

	Immunoassay by graphite furnace atomic absorption spectrometry using a metal chelate as a label
	Wang, GL ; Yuan, JL ; Gong, BL ; Matsumoto, K ; Hu, ZD
刊名	analytica chimica acta
	2001-12-03
卷号	448 期号:1-2 页码:165-172
关键词	immunoassay atomic absorption spectrometry alpha-fetoprotein bensulfuron-methyl
英文摘要	a new immunoassay method was developed by using graphite furnace atomic absorption spectrometry and edta-cd2+ chelate as a label. the new approach combined the use of edta-cd2+ chelate labeled streptavidin (sa), biotinylated antibody or antigen, and graphite furnace atomic absorption detection for both sandwich-type immunoassay and competitive-type immunoassay. the measurements of alpha -fetoprotein (afp) in human sera and bensulfuron-methyl (bsm) in water were used as the models to evaluate the usefulness of the method. the assays were carried out with 96-well microtiter plate as solid-phase carrier. after the immune reaction and biotin-streptavidin binding reaction, a solution of 0.2 m hno3 was added to each well to dissociate cd2+ in the immune complex on the solid-phase into the solution. the antigen concentration, was thus, determined by measuring the cd2+ absorbance of the solution with graphite furnace atomic absorption spectrometry. the present method gives detection limits of 0.12 ng ml(-1) for afp and 0.95 ng ml(-1) for bsm, respectively. the coefficient variations (cvs) of the method are less than 8.0%, and the recoveries are in the range of 90-110%. the concentrations of afp in 23 human serum samples were determined, and the results were compared with those of the independently determined by time-resolved fluoroimmunoassay method. a good correlation was obtained with a correlation coefficient of 0.993. (c) 2001 elsevier science b.v. all rights reserved.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	time-resolved fluorescence ; alpha-fetoprotein ; biotechnology
收录类别	SCI
语种	英语
WOS记录号	WOS:000172458400018
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/138943]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116012, Peoples R China 2.Lanzhou Univ, Dept Chem, Lanzhou 730001, Peoples R China 3.Waseda Univ, Dept Chem, Shinjuku Ku, Tokyo 1698555, Japan
推荐引用方式 GB/T 7714	Wang, GL,Yuan, JL,Gong, BL,et al. Immunoassay by graphite furnace atomic absorption spectrometry using a metal chelate as a label[J]. analytica chimica acta,2001,448(1-2):165-172.
APA	Wang, GL,Yuan, JL,Gong, BL,Matsumoto, K,&Hu, ZD.(2001).Immunoassay by graphite furnace atomic absorption spectrometry using a metal chelate as a label.analytica chimica acta,448(1-2),165-172.
MLA	Wang, GL,et al."Immunoassay by graphite furnace atomic absorption spectrometry using a metal chelate as a label".analytica chimica acta 448.1-2(2001):165-172.