Homogeneous time-resolved fluorescence DNA hybridization assay by DNA-mediated formation of an EDTA-Eu(III)-beta-diketonate ternary complex

	Homogeneous time-resolved fluorescence DNA hybridization assay by DNA-mediated formation of an EDTA-Eu(III)-beta-diketonate ternary complex
	Wang, GL ; Yuan, JL ; Matsumoto, K ; Hu, Z
刊名	analytical biochemistry
	2001-12-15
卷号	299 期号:2 页码:169-172
英文摘要	a sensitive homogenous time-resolved fluorescence dna hybridization assay method based on the formation of an edta-eu3+-beta-diketonate ternary complex in the dna hybrid was developed. the new approach combined the use of two dna probes whose sequences compose the whole complementary strand to the target dna, in which one probe was labeled with an edta-eu3+ complex on the 5'-terminus and the other, labeled with a bidentate beta-diketone on the 3'-terminus. after hybridization of two dna probes with target dna, edta-eu3+ and beta-diketone come close to each other, and an edta-eu3+-beta-diketonate ternary complex with a strong and long-lived fluorescence was formed; thus the target dna was detected sensitively with a detection limit of 6 pm (0.6 fmol per assay) by time-resolved fluorescence measurement. in the absence of the target dna, due to the poor stability of bidentate beta-diketonate-eu3+ complex in very diluted solution, only a small amount of ternary fluorescence complex was formed. (c) 2001 elsevier science.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; biochemistry & molecular biology ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	energy-transfer ; biotechnology ; probes ; gene
收录类别	SCI
语种	英语
WOS记录号	WOS:000173223300007
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/138909]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Lanzhou Univ, Dept Chem, Lanzhou 730001, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116012, Peoples R China 3.Waseda Univ, Dept Chem, Shinjuku Ku, Tokyo 1698555, Japan
推荐引用方式 GB/T 7714	Wang, GL,Yuan, JL,Matsumoto, K,et al. Homogeneous time-resolved fluorescence DNA hybridization assay by DNA-mediated formation of an EDTA-Eu(III)-beta-diketonate ternary complex[J]. analytical biochemistry,2001,299(2):169-172.
APA	Wang, GL,Yuan, JL,Matsumoto, K,&Hu, Z.(2001).Homogeneous time-resolved fluorescence DNA hybridization assay by DNA-mediated formation of an EDTA-Eu(III)-beta-diketonate ternary complex.analytical biochemistry,299(2),169-172.
MLA	Wang, GL,et al."Homogeneous time-resolved fluorescence DNA hybridization assay by DNA-mediated formation of an EDTA-Eu(III)-beta-diketonate ternary complex".analytical biochemistry 299.2(2001):169-172.