Study of enantioselective interactions between chiral drugs and serum albumin by capillary electrophoresis

	Study of enantioselective interactions between chiral drugs and serum albumin by capillary electrophoresis
	Zhu, XF ; Ding, YS ; Lin, BC ; Jakob, A ; Koppenhoefer, B
刊名	electrophoresis
	1999-07-01
卷号	20 期号:9 页码:1869-1877
关键词	capillary electrophoresis chiral separation protein interaction stereoselectivity
英文摘要	the separation of the enantiomers of three basic drugs, i.e., ofloxacin, propranolol and verapamil, was achieved by affinity capillary electrophoresis (ace), with human serum albumin (hsa) and bovine serum albumin (bsa) as chiral selectors in phosphate buffer at ph 7.4. ofloxacin was only separated in the presence of bsa, and verapamil only with hsa, while propranolol was separated with either hsa or bsa. the effects of protein concentration and column wall adsorption on the degree of separation were investigated. two displacers, ketoprofen and warfarin, respectively, when added to the protein containing buffer, both showed significant effects on the separation behavior. from these data it was argued that verapamil may bind to hsa at both locations known, the warfarin binding site (i) and the ketoprofen binding site (ii). while with bsa, binding of ofloxacin may also occur at site i, the preferential binding site for propanolol remains controversial. a drug-drug interaction between propranolol and ketoprofen due to opposite charges was concluded from the increase in migration time in bsa solution. the unbound concentration of verapamil enantiomers in solution in the presence of hsa, as estimated from cd-modified capillary zone electrophoresis, was triggered not only by the hsa concentration but also by the coadditive concentration.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	partial-filling technique ; affinity electrokinetic chromatography ; human alpha(1)-acid glycoprotein ; zone-electrophoresis ; protein-binding ; stereoselective binding ; ketoprofen enantiomers ; frontal analysis ; solvating agent ; basic drug
收录类别	ISTP ; SCI
语种	英语
WOS记录号	WOS:000081688800011
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/138652]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Univ Tubingen, Inst Organ Chem, D-72074 Tubingen, Germany
推荐引用方式 GB/T 7714	Zhu, XF,Ding, YS,Lin, BC,et al. Study of enantioselective interactions between chiral drugs and serum albumin by capillary electrophoresis[J]. electrophoresis,1999,20(9):1869-1877.
APA	Zhu, XF,Ding, YS,Lin, BC,Jakob, A,&Koppenhoefer, B.(1999).Study of enantioselective interactions between chiral drugs and serum albumin by capillary electrophoresis.electrophoresis,20(9),1869-1877.
MLA	Zhu, XF,et al."Study of enantioselective interactions between chiral drugs and serum albumin by capillary electrophoresis".electrophoresis 20.9(1999):1869-1877.