Bio-inspired protein-gold nanoconstruct with core-void-shell structure: beyond a chemo drug carrier

	Bio-inspired protein-gold nanoconstruct with core-void-shell structure: beyond a chemo drug carrier
	Liu, Xiangyou 1,6; Wei, Wei 2; Huang, Shijiao 3,4; Lin, Shrong-Shi 1; Zhang, Xin 5; Zhang, Chuanmao 3,4; Du, Yuguang 6; Ma, Guanghui 2; Li, Mei 7; Mann, Stephen 7
刊名	journal of materials chemistry b
	2013
卷号	1 期号:25 页码:3136-3143
英文摘要	chemotherapy has been widely used in clinical practice for cancer treatment. a major challenge for a successful chemotherapy is to potentiate the anticancer activity, whilst reducing the severe side effects. in this context, we design a bio-inspired protein-gold nanoconstruct (denoted as aft-au hereafter) with a core-void-shell structure which exhibits a high selectivity towards carcinoma cells. anticancer drug 5-fluorouracil (5-fu) can be sequestered into the void space of the construct to produce an integrated nanoscale hybrid aft-aufu that exhibits an increased cellular uptake of 5-fu. more importantly, aft-au, serving as a bio-nano-chemosensitizer, renders carcinoma cells more susceptible to 5-fu by cell-cycle regulation, and thus, leads to a dramatic decrease of the ic50 value (i.e. the drug concentration required to kill 50% of the cell population) of 5-fu in hepg2 cells from 138.3 mu m to 9.2 mu m. besides hepg2 cells, a remarkably enhanced anticancer efficacy and potentially reduced side effects are also achieved in other cell lines. our further work reveals that the drug 5-fu is internalized into cells with aft-au primarily via receptor-mediated endocytosis (rme). after internalization, aft-aufu colocalizes with lysosomes which trigger the release of 5-fu under acidic conditions. overall, our approach provides a novel procedure in nanoscience that promises an optimal chemotherapeutic outcome.
WOS标题词	science & technology ; technology
类目[WOS]	materials science, biomaterials
研究领域[WOS]	materials science
关键词[WOS]	horse spleen apoferritin ; cancer-cells ; ferritin receptors ; anticancer drugs ; phase-transition ; cellular uptake ; iron release ; cyclin-e ; nanoparticles ; 5-fluorouracil
收录类别	SCI
语种	英语
WOS记录号	WOS:000319965800002
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/137948]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Peking Univ, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 3.Peking Univ, MOE Key Lab Cell Proliferat & Differentiat, Coll Life Sci, Beijing 100871, Peoples R China 4.Peking Univ, State Key Lab Biomembrane & Membrane Biotechnol, Coll Life Sci, Beijing 100871, Peoples R China 5.Scripps Res Inst, Dept Mol & Expt Med, La Jolla, CA 92037 USA 6.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 7.Univ Bristol, Sch Chem, Ctr Organized Matter Chem, Bristol BS6 1TS, Avon, England
推荐引用方式 GB/T 7714	Liu, Xiangyou,Wei, Wei,Huang, Shijiao,et al. Bio-inspired protein-gold nanoconstruct with core-void-shell structure: beyond a chemo drug carrier[J]. journal of materials chemistry b,2013,1(25):3136-3143.
APA	Liu, Xiangyou.,Wei, Wei.,Huang, Shijiao.,Lin, Shrong-Shi.,Zhang, Xin.,...&Ma, Ding.(2013).Bio-inspired protein-gold nanoconstruct with core-void-shell structure: beyond a chemo drug carrier.journal of materials chemistry b,1(25),3136-3143.
MLA	Liu, Xiangyou,et al."Bio-inspired protein-gold nanoconstruct with core-void-shell structure: beyond a chemo drug carrier".journal of materials chemistry b 1.25(2013):3136-3143.