Proliferation, Sternness and Cardiac Differentiation Properties of Umbilical Cord Wharton's Jelly-derived Mesenchymal Stem Cells during the Continuous Passages

	Proliferation, Sternness and Cardiac Differentiation Properties of Umbilical Cord Wharton's Jelly-derived Mesenchymal Stem Cells during the Continuous Passages
	Lv, Shi-jie 1,5; Liu, Yang 2; Lian, Jian-chun 3; He, Xin 3; Liu, Chang 2,4; Sun, Guang-wei 2; Guo, Xin 2; Ma, Xiao-jun 2; Li, Hui 1
刊名	journal of hard tissue biology
	2013-04-01
卷号	22 期号:2 页码:261-266
关键词	Cardiac differentiation Continuous passage Fetal mesenchymal stem cell Stemness
英文摘要	fetal tissue-derived mesenchymal stem cells (mscs) may offer greater clinical utility because they possess unique properties over bone marrow (bm) mscs. although the continuous passage process can generate numerous mscs for clinical applications, this process may also have an important effect on the bological characteristics of fetal mscs. in this study, the proliferation, sternness and cardiac differentiation properties of umbilical cord (uc) wharton's jelly-derived mscs (wj mscs) at different passages (3, 7 and 14) was investigated. it was found that wj msc proliferation rate was decreased during the continuous passage process. and the esc marker gene (oct3/4, nanog) expression in wj mscs also decreased gradually, while the early mesoderm marker gene (vimentin) was highest at passage 7. moreover, the cardiac marker gene (nkx2.5) expression in differentiated mscs increased during the continuous passage after cardiac induction. our results may provide some useful information for stem cell therapy based on wj mscs.
WOS标题词	science & technology ; technology
类目[WOS]	engineering, biomedical
研究领域[WOS]	engineering
关键词[WOS]	myocardial-infarction ; bone-marrow
收录类别	SCI
语种	英语
WOS记录号	WOS:000318190200016
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/137896]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.China Med Univ, Dept Obstet & Gynecol, Shengjing Hosp, Shenyang, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian, Peoples R China 3.Dalian Med Univ, Lab Med Coll, Dalian, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Dalian Matern Hosp, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Lv, Shi-jie,Liu, Yang,Lian, Jian-chun,et al. Proliferation, Sternness and Cardiac Differentiation Properties of Umbilical Cord Wharton's Jelly-derived Mesenchymal Stem Cells during the Continuous Passages[J]. journal of hard tissue biology,2013,22(2):261-266.
APA	Lv, Shi-jie.,Liu, Yang.,Lian, Jian-chun.,He, Xin.,Liu, Chang.,...&Li, Hui.(2013).Proliferation, Sternness and Cardiac Differentiation Properties of Umbilical Cord Wharton's Jelly-derived Mesenchymal Stem Cells during the Continuous Passages.journal of hard tissue biology,22(2),261-266.
MLA	Lv, Shi-jie,et al."Proliferation, Sternness and Cardiac Differentiation Properties of Umbilical Cord Wharton's Jelly-derived Mesenchymal Stem Cells during the Continuous Passages".journal of hard tissue biology 22.2(2013):261-266.