Full-color structured illumination optical sectioning microscopy

CORC > 西安光学精密机械研究所 > 中国科学院西安光学精密机械研究所 > 瞬态光学技术国家重点实验室

	Full-color structured illumination optical sectioning microscopy
	Qian, Jia; Lei, Ming; Dan, Dan; Yao, Baoli; Zhou, Xing; Yang, Yanlong; Yan, Shaohui; Min, Junwei; Yu, Xianghua
刊名	scientific reports
	2015-09-29
卷号	5
英文摘要	in merits of super-resolved resolution and fast speed of three-dimensional (3d) optical sectioning capability, structured illumination microscopy (sim) has found variety of applications in biomedical imaging. so far, most sim systems use monochrome ccd or cmos cameras to acquire images and discard the natural color information of the specimens. although multicolor integration scheme are employed, multiple excitation sources and detectors are required and the spectral information is limited to a few of wavelengths. here, we report a new method for full-color sim with a color digital camera. a data processing algorithm based on hsv (hue, saturation, and value) color space is proposed, in which the recorded color raw images are processed in the hue, saturation, value color channels, and then reconstructed to a 3d image with full color. we demonstrated some 3d optical sectioning results on samples such as mixed pollen grains, insects, micro-chips and the surface of coins. the presented technique is applicable to some circumstance where color information plays crucial roles, such as in materials science and surface morphology.
WOS标题词	science & technology
类目[WOS]	multidisciplinary sciences
研究领域[WOS]	science & technology - other topics
关键词[WOS]	3-d shape measurement ; fluorescence microscopy ; coherence tomography ; plane illumination ; superresolution ; light ; cells ; resolution
收录类别	SCI
语种	英语
WOS记录号	WOS:000361878800002
公开日期	2015-11-03
内容类型	期刊论文
源URL	[http://ir.opt.ac.cn/handle/181661/25402]
专题	西安光学精密机械研究所_瞬态光学技术国家重点实验室
作者单位	Chinese Acad Sci, State Key Lab Transient Opt & Photon, Xian Inst Opt & Precis Mech, Xian 710119, Peoples R China
推荐引用方式 GB/T 7714	Qian, Jia,Lei, Ming,Dan, Dan,et al. Full-color structured illumination optical sectioning microscopy[J]. scientific reports,2015,5.
APA	Qian, Jia.,Lei, Ming.,Dan, Dan.,Yao, Baoli.,Zhou, Xing.,...&Yu, Xianghua.(2015).Full-color structured illumination optical sectioning microscopy.scientific reports,5.
MLA	Qian, Jia,et al."Full-color structured illumination optical sectioning microscopy".scientific reports 5(2015).