Basic Principle of SLS/DLS Measurements in Fluorescent/Phosphorescence Solutions

	Basic Principle of SLS/DLS Measurements in Fluorescent/Phosphorescence Solutions
	Jia, Di 1; Yang, Jing-fa 1; Cheng, He 2,3; Han, Charles C.1
刊名	ACTA POLYMERICA SINICA
	2015-05-20
期号	5 页码:564-570
关键词	Laser light scattering Fluorescent/phosphorescence Fluorescence correlation spectroscopy (FCS) Quasi-elastic scattering
英文摘要	As a typical elastic scattering technique,laser light scattering results cannot precisely describe the solution structure when the samples absorb incident light. Therefore, commercial laser light scattering instrument has been modified and upgraded to utilize four independent lasers, e. g. 785 nm,632.8 nm,532 nm and 457 nm, respectively as the light sources. By switching the wavelengths of incident lasers to avoid the sample absorption, the instrument can be widely used in fluorescent/phosphorescence solution systems. Spherical polystyrene nanoparticles with and without fluorescent dyes are used to calibrate the modified instrument. It is found that the height of the normalized intensity-intensity time correlation function decreases with the increase of the scattering angle when the sample absorbs the incident laser light. Such a correlation function fails to describe the sample solution structure, because the corresponding hydrodynamic radius distribution shows two modes and the average characteristic line width versus the square of scattering vector q(2) does not fit a good linear relationship any more. Therefore, another laser which is not absorbed by the sample is used to do the calibration again. The corresponding intensity-intensity time correlation functions and the fitting results can precisely describe the solution structure. Fluorescence correlation spectroscopy (FCS) is also conducted to further prove the results obtained from the modified laser light scattering instrument are precise and accurate.
收录类别	SCI
语种	英语
公开日期	2015-11-02
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/28162]
专题	化学研究所_高分子物理与化学实验室
作者单位	1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, State Key Lab Polymer Phys & Chem, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst High Energy Phys, China Spallat Neutron Source, Dongguan 523803, Peoples R China 3.Dongguan Inst Neutron Sci, Dongguan 523808, Peoples R China
推荐引用方式 GB/T 7714	Jia, Di,Yang, Jing-fa,Cheng, He,et al. Basic Principle of SLS/DLS Measurements in Fluorescent/Phosphorescence Solutions[J]. ACTA POLYMERICA SINICA,2015(5):564-570.
APA	Jia, Di,Yang, Jing-fa,Cheng, He,&Han, Charles C..(2015).Basic Principle of SLS/DLS Measurements in Fluorescent/Phosphorescence Solutions.ACTA POLYMERICA SINICA(5),564-570.
MLA	Jia, Di,et al."Basic Principle of SLS/DLS Measurements in Fluorescent/Phosphorescence Solutions".ACTA POLYMERICA SINICA .5(2015):564-570.