PEGylated poly(aspartate-g-OEI) copolymers for effective and prolonged gene transfection

	PEGylated poly(aspartate-g-OEI) copolymers for effective and prolonged gene transfection
	Feng,Tianshi ; Dong,Xuan ; Tian,Huayu ; Lam,Michael Hon-Wah ; Liang,Haojun ; Wei,Yen ; Chen,Xuesi
刊名	journal of materials chemistry b
	2014
卷号	2 期号:18 页码:2725-2732
关键词	PLASMID DNA DELIVERY IN-VIVO MOLECULAR-WEIGHT DRUG-DELIVERY THERAPY DEATH NANOPARTICLES POLYETHYLENIMINE EFFICIENCY MICELLES CARRIERS
通讯作者	feng,ts
英文摘要	two pegylated poly(aspartate-g-oei) cationic copolymers, peg-b-pasp-g-oei (dao) and oei-g-pasp-b-peg-b-pasp-g-oei (tao), were developed for in vivo gene transfer, and a non-pegylated copolymer (mao) was utilized as a control. these copolymers exhibited favorable capacities for condensing plasmid dna (pdna) into nanosized particles (90-180 nm) with positive surface charges. gene transfection efficiency of the copolymers (especially dao) demonstrated improved performance compared to pei25k in both hela and hek 293 cell lines in the presence of serum. although mao and dao show similar gene transfection efficiency in vitro, dao is shown to be more effective in vivo. the potential reason is that pegylation enhances the serum-resistance of the carriers and prolongs gene transfection in vivo. for tao, despite its peg segment, the complex of copolymer-pdna is encompassed by a cation shell and cannot reduce the serum effects. these results suggest that pegylated diblock copolymers have potential as non-viral gene carriers in gene delivery systems for in vivo application.
收录类别	SCI
语种	英语
公开日期	2015-10-27
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/56216]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Feng,Tianshi,Dong,Xuan,Tian,Huayu,et al. PEGylated poly(aspartate-g-OEI) copolymers for effective and prolonged gene transfection[J]. journal of materials chemistry b,2014,2(18):2725-2732.
APA	Feng,Tianshi.,Dong,Xuan.,Tian,Huayu.,Lam,Michael Hon-Wah.,Liang,Haojun.,...&Chen,Xuesi.(2014).PEGylated poly(aspartate-g-OEI) copolymers for effective and prolonged gene transfection.journal of materials chemistry b,2(18),2725-2732.
MLA	Feng,Tianshi,et al."PEGylated poly(aspartate-g-OEI) copolymers for effective and prolonged gene transfection".journal of materials chemistry b 2.18(2014):2725-2732.