Analysis of human urine metabolites using SPE and NMR spectroscopy

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 2011年以前论文发表（包括2011年）

	Analysis of human urine metabolites using SPE and NMR spectroscopy
	Yang WeiJin 1; Wang YaWei 1; Zhou QunFang 1; Tang HuiRu 2
刊名	SCIENCE IN CHINA SERIES B-CHEMISTRY
	2008-03-01
卷号	51 期号:3 页码:218-225
关键词	urine metabolites NMR spectroscopy solid phase extraction metabonomics metabolomics
英文摘要	Nuclear magnetic resonance (NMR) spectroscopic analysis of metabonome/metabolome has wide-spread applications in biomedical science researches. However, most of NMR resonances for urinary metabolites remain to be fully assigned. In the present study, human urine samples from two healthy volunteers were pre-treated with C18 solid-phase extraction and the resultant 5 sub-fractions were subjected to one-and two-dimensional NMR studies, including (1)H J-Resolved, (1)H-(1)H COSY, (1)H-(1)H TOCSY, (1)H-(13)C HSQC, and HMBC 2D NMR. More than 70 low molecular weight metabolites were identified, and complete assignments of (1)H and (13)C resonances including many complex coupled spin systems were obtained.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Multidisciplinary
研究领域[WOS]	Chemistry
关键词[WOS]	SPINNING H-1-NMR SPECTROSCOPY ; CHROMATOGRAPHY-MASS SPECTROMETRY ; MAGNETIC-RESONANCE-SPECTROSCOPY ; MULTIVARIATE DATA-ANALYSIS ; MATRICARIA-RECUTITA L. ; ALLYL FORMATE ; BLOOD-PLASMA ; UPLC-MS ; METABONOMICS ; MODEL
收录类别	SCI
语种	英语
WOS记录号	WOS:000253204200004
公开日期	2015-10-13
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/7990]
专题	武汉物理与数学研究所_2011年以前论文发表（包括2011年）
作者单位	1.Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Yang WeiJin,Wang YaWei,Zhou QunFang,et al. Analysis of human urine metabolites using SPE and NMR spectroscopy[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2008,51(3):218-225.
APA	Yang WeiJin,Wang YaWei,Zhou QunFang,&Tang HuiRu.(2008).Analysis of human urine metabolites using SPE and NMR spectroscopy.SCIENCE IN CHINA SERIES B-CHEMISTRY,51(3),218-225.
MLA	Yang WeiJin,et al."Analysis of human urine metabolites using SPE and NMR spectroscopy".SCIENCE IN CHINA SERIES B-CHEMISTRY 51.3(2008):218-225.