Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry

	Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry
	Kang TF(康腾飞); Niu Y(牛宇); Jin G(靳刚)
	2014
会议名称	6th International Conference on Spectroscopic Ellipsometry
会议日期	MAY 26-31 2013
会议地点	Kyoto JAPAN
关键词	Biosensor Total internal reflection imaging ellipsometry Weak affinity interaction Tris Lysozyme
通讯作者	gajin@imech.ac.cn
中文摘要	Weak affinity interactions among biomolecules have attracted more and more attentions for they play a significant role in organisms. However it is difficult to characterize these interactions due to the limitation of the detection approaches. The biosensor based on total internal reflection imaging ellipsometry is a real-time analysis method for biomolecular interaction detection with the advantages of high throughput and sensitivity. The interaction between tris and lysozyme a typical example of weak affinity interactions has been detected with TIRIE as a trial. Tris is immobilized on the gold thin film substrate to form the biosensing surface and then LZM as well as its negative controls is delivered to the tris-immobilized surface. The interaction process between tris and LZM is recorded by TIRIE biosensor to forma real-time curve and its dissociation constant is deduced as 7.6 x 10 M which is in agreement with the results in the reported work by other investigators. The results indicate that TIRIE biosensor is competent for weak affinity interaction analysis. 2014 Elsevier B.V. All rights reserved.
收录类别	SCI ; CPCI
会议网址	http://dx.doi.org/10.1016/j.tsf.2014.02.098
会议录	THIN SOLID FILMS
会议录出版者	ELSEVIER SCIENCE SA
会议录出版地	LAUSANNE
语种	英语
内容类型	会议论文
源URL	[http://dspace.imech.ac.cn/handle/311007/55365]
专题	力学研究所_国家微重力实验室
推荐引用方式 GB/T 7714	Kang TF,Niu Y,Jin G. Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry[C]. 见:6th International Conference on Spectroscopic Ellipsometry. Kyoto JAPAN. MAY 26-31 2013.http://dx.doi.org/10.1016/j.tsf.2014.02.098.