Antioxidative responses in zebrafish liver exposed to sublethal doses Aphanizomenon flos-aquae DC-1 aphantoxins

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	Antioxidative responses in zebrafish liver exposed to sublethal doses Aphanizomenon flos-aquae DC-1 aphantoxins
	Zhang, De Lu 1; Liu, Si Yi 1; Zhang, Jing 2; Hu, Chun Xiang 3; Li, Dun Hai 3; Liu, Yong Ding 3
刊名	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
	2015-03-01
卷号	113 页码:425-432
关键词	Cyanobacterial neurotoxins Zebrafish liver Antioxidative response Biomarkers Hepatotoxicity
ISSN号	0147-6513
通讯作者	Hu, CX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
英文摘要	Aphanizomenon flos-aquae secretes paralytic shellfish poisons (PSPs), termed aphantoxins, and endangers environmental and human health via eutrophication of water worldwide. Although the molecular mechanism of neuronal PSP toxicity has been well studied, several issues remain unresolved, notably the in vivo hepatic antioxidative responses to this neurotoxin. Aphantoxins extracted from a natural isolate of A. flos-aquae DC-1 were resolved by high performance liquid chromatography. The primary components were gonyautoxins 1 and 5 and neosaxitoxin. Zebrafish (Danio rerio) were treated intraperitoneally with either 53 or 7.61 (low and high doses, respectively) mu g saxitoxin (STX) equivalents (eq)/kg of A. flos-aquae DC-1 aphantoxins. Antioxidative responses in zebrafish liver were examined at different timepoints 1-24 h post-exposure. Aphantoxin administration significantly enhanced hepatic malondialdehyde (MDA) content 1-12 h post-exposure, indicative of oxidative stress and lipid peroxidation. By contrast, levels of reduced glutathione (GSH) in zebrafish liver declined significantly after 3-24 h exposure, suggesting that GSH participates in MDA metabolism. A significant upregulation of the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) was observed, suggesting that aphantoxins induce lipid peroxidation in zebrafish liver and are likely to be hepatotoxic. Hepatic levels of MDA and GSH, and of the three enzymes (SOD, CAT, and GPx), therefore provide potential biomarkers for studying environmental exposure to aphantoxins/PSPs from cyanobacterial blooms. (C) 2014 Elsevier Inc. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Environmental Sciences ; Toxicology
研究领域[WOS]	Environmental Sciences & Ecology ; Toxicology
关键词[WOS]	TILAPIA OREOCHROMIS-NILOTICUS ; SUPEROXIDE-DISMUTASE SOD ; OXIDATIVE STRESS ; MICROCYSTIN-LR ; LIPID-PEROXIDATION ; GLUTATHIONE-PEROXIDASE ; LABORATORY CONDITIONS ; CYANOBACTERIAL BLOOM ; TOXIC DINOFLAGELLATE ; AGGRESSIVE-BEHAVIOR
收录类别	SCI
语种	英语
WOS记录号	WOS:000348966400050
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/25774]
专题	水生生物研究所_藻类生物学及应用研究中心_期刊论文
作者单位	1.Wuhan Univ Technol, Sch Chem Chem Engn & Life Sci, Dept Lifesci & Biotechnol, Wuhan 430070, Peoples R China 2.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Zhang, De Lu,Liu, Si Yi,Zhang, Jing,et al. Antioxidative responses in zebrafish liver exposed to sublethal doses Aphanizomenon flos-aquae DC-1 aphantoxins[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2015,113:425-432.
APA	Zhang, De Lu,Liu, Si Yi,Zhang, Jing,Hu, Chun Xiang,Li, Dun Hai,&Liu, Yong Ding.(2015).Antioxidative responses in zebrafish liver exposed to sublethal doses Aphanizomenon flos-aquae DC-1 aphantoxins.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,113,425-432.
MLA	Zhang, De Lu,et al."Antioxidative responses in zebrafish liver exposed to sublethal doses Aphanizomenon flos-aquae DC-1 aphantoxins".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 113(2015):425-432.