Characterization of the hypoxia-inducible factor 1 alpha gene in the sperm whale, beluga whale, and Yangtze finless porpoise

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物多样性与资源保护研究中心 > 期刊论文

	Characterization of the hypoxia-inducible factor 1 alpha gene in the sperm whale, beluga whale, and Yangtze finless porpoise
	Bi, Jianling 1,2; Hu, Bo 1; Zheng, Jinsong 1; Wang, Jingzhen 1; Xiao, Wuhan 1; Wang, Ding 1
刊名	MARINE BIOLOGY
	2015-06-01
卷号	162 期号:6 页码:1201-1213
ISSN号	0025-3162
通讯作者	Xiao, WH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China.
英文摘要	Cetaceans have evolved many behavioral and biological adaptations to allow them to survive in aquatic environments. Because of diving and breath-holding behaviors, cetaceans experience wide fluctuations in oxygen tension. However, the molecular basis behind their adaptation to a wide range of diving depths and durations remains unknown. Hypoxia-inducible factor (HIF) is a crucial regulator of cellular and systemic responses to low oxygen levels. In this study, the HIF-1 alpha gene was cloned from the sperm whale (Physeter macrocephalus), the beluga whale (Delphinapterus leucas), and the Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis). HIF-1 alpha from the deep-diving sperm whale degraded rapidly under normoxia but remained stable during hypoxia compared with HIF-1 alpha from the beluga whale and the Yangtze finless porpoise. Sperm whale HIF-1 alpha also responded to reoxygenation significantly faster and exerted more potent effects on the transcriptional activity of some gene promoters compared with HIF-1 alpha from the beluga whale and the Yangtze finless porpoise. Mutations in HIF-1 alpha were detected between the Yangtze finless porpoise and the sperm whale, which may determine their functional differences. The results suggest that the response of sperm whale HIF-1 alpha to different oxygen tensions is more variable than that of Yangtze finless porpoise or beluga whale HIF-1 alpha, indicating that the rate of degradation of HIF-1 alpha may be more variable in long-duration divers than in short-duration diver cetaceans. Because of the lack of whale models and whale cell lines, all experiments were conducted in vitro using human embryonic kidney 293T cells.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Marine & Freshwater Biology
研究领域[WOS]	Marine & Freshwater Biology
关键词[WOS]	ELEPHANT SEAL PUPS ; DIVE-ASSOCIATED ISCHEMIA/REPERFUSION ; GROWTH-FACTOR EXPRESSION ; RINGED SEAL ; OXIDATIVE STRESS ; FACTOR-I ; CLONING ; CETACEANS ; TISSUES ; PROLYL
收录类别	SCI
语种	英语
WOS记录号	WOS:000354619600005
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/23823]
专题	水生生物研究所_水生生物多样性与资源保护研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Bi, Jianling,Hu, Bo,Zheng, Jinsong,et al. Characterization of the hypoxia-inducible factor 1 alpha gene in the sperm whale, beluga whale, and Yangtze finless porpoise[J]. MARINE BIOLOGY,2015,162(6):1201-1213.
APA	Bi, Jianling,Hu, Bo,Zheng, Jinsong,Wang, Jingzhen,Xiao, Wuhan,&Wang, Ding.(2015).Characterization of the hypoxia-inducible factor 1 alpha gene in the sperm whale, beluga whale, and Yangtze finless porpoise.MARINE BIOLOGY,162(6),1201-1213.
MLA	Bi, Jianling,et al."Characterization of the hypoxia-inducible factor 1 alpha gene in the sperm whale, beluga whale, and Yangtze finless porpoise".MARINE BIOLOGY 162.6(2015):1201-1213.