Bacterial communities in haloalkaliphilic sulfate-reducing bioreactors under different electron donors revealed by 16S rRNA MiSeq sequencing

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	Bacterial communities in haloalkaliphilic sulfate-reducing bioreactors under different electron donors revealed by 16S rRNA MiSeq sequencing
	Zhou, Jiemin 1,2; Zhou, Xuemei 3; Li, Yuguang 3; Xing, Jianmin 1
刊名	JOURNAL OF HAZARDOUS MATERIALS
	2015-09-15
卷号	295 期号:SEP 页码:176-184
关键词	Haloalkaliphilic Sulfate reduction MiSeq Bacterial community
ISSN号	0304-3894
英文摘要	Biological technology used to treat flue gas is useful to replace conventional treatment, but there is sulfide inhibition. However, no sulfide toxicity effect was observed in haloalkaliphilic bioreactors. The performance of the ethanol-fed bioreactor was better than that of lactate-, glucose-, and formate-fed bioreactor, respectively. To support this result strongly, Illumina MiSeq paired-end sequencing of 16S rRNA gene was applied to investigate the bacterial communities. A total of 389,971 effective sequences were obtained and all of them were assigned to 10,220 operational taxonomic units (OTUs) at a 97% similarity. Bacterial communities in the glucose-fed bioreactor showed the greatest richness and evenness. The highest relative abundance of sulfate-reducing bacteria (SRB) was found in the ethanol-fed bioreactor, which can explain why the performance of the ethanol-fed bioreactor was the best. Different types of SRB, sulfur-oxidizing bacteria, and sulfur-reducing bacteria were detected, indicating that sulfur may be cycled among these microorganisms. Because high-throughput 16S rRNA gene paired-end sequencing has improved resolution of bacterial community analysis, many rare microorganisms were detected, such as Halanaerobium, Halothiobacillus, Desulfonatronum, Syntrophobacter, and Fusibacter. 16S rRNA gene sequencing of these bacteria would provide more functional and phylogenetic information about the bacterial communities. (C) 2015 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Technology ; Life Sciences & Biomedicine
类目[WOS]	Engineering, Environmental ; Engineering, Civil ; Environmental Sciences
研究领域[WOS]	Engineering ; Environmental Sciences & Ecology
关键词[WOS]	PURPLE NONSULFUR BACTERIUM ; THIODISMUTANS SP NOV. ; WATER TREATMENT-PLANT ; SODA LAKES ; GEN. NOV ; FLUE-GAS ; REDUCTION ; PERFORMANCE ; DIVERSITY ; GROWTH
收录类别	SCI
语种	英语
WOS记录号	WOS:000356127400023
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/19284]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Minist Civil Affairs, Inst 101, Beijing 100070, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Jiemin,Zhou, Xuemei,Li, Yuguang,et al. Bacterial communities in haloalkaliphilic sulfate-reducing bioreactors under different electron donors revealed by 16S rRNA MiSeq sequencing[J]. JOURNAL OF HAZARDOUS MATERIALS,2015,295(SEP):176-184.
APA	Zhou, Jiemin,Zhou, Xuemei,Li, Yuguang,&Xing, Jianmin.(2015).Bacterial communities in haloalkaliphilic sulfate-reducing bioreactors under different electron donors revealed by 16S rRNA MiSeq sequencing.JOURNAL OF HAZARDOUS MATERIALS,295(SEP),176-184.
MLA	Zhou, Jiemin,et al."Bacterial communities in haloalkaliphilic sulfate-reducing bioreactors under different electron donors revealed by 16S rRNA MiSeq sequencing".JOURNAL OF HAZARDOUS MATERIALS 295.SEP(2015):176-184.