Analysis of human urine metabolites using SPE and NMR spectroscopy

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Analysis of human urine metabolites using SPE and NMR spectroscopy
	Yang WeiJin ; Wang YaWei ; Zhou QunFang ; Tang HuiRu
刊名	SCIENCE IN CHINA SERIES B-CHEMISTRY
	2008-03
卷号	51 期号:3 页码:218-225
关键词	urine metabolites NMR spectroscopy solid phase extraction metabonomics metabolomics
英文摘要	Nuclear magnetic resonance (NMR) spectroscopic analysis of metabonome/metabolome has wide-spread applications in biomedical science researches. However, most of NMR resonances for urinary metabolites remain to be fully assigned. In the present study, human urine samples from two healthy volunteers were pre-treated with C18 solid-phase extraction and the resultant 5 sub-fractions were subjected to one-and two-dimensional NMR studies, including (1)H J-Resolved, (1)H-(1)H COSY, (1)H-(1)H TOCSY, (1)H-(13)C HSQC, and HMBC 2D NMR. More than 70 low molecular weight metabolites were identified, and complete assignments of (1)H and (13)C resonances including many complex coupled spin systems were obtained.
WOS记录号	WOS:000253204200004
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/21911]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Yang WeiJin,Wang YaWei,Zhou QunFang,et al. Analysis of human urine metabolites using SPE and NMR spectroscopy[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2008,51(3):218-225.
APA	Yang WeiJin,Wang YaWei,Zhou QunFang,&Tang HuiRu.(2008).Analysis of human urine metabolites using SPE and NMR spectroscopy.SCIENCE IN CHINA SERIES B-CHEMISTRY,51(3),218-225.
MLA	Yang WeiJin,et al."Analysis of human urine metabolites using SPE and NMR spectroscopy".SCIENCE IN CHINA SERIES B-CHEMISTRY 51.3(2008):218-225.