DNA wrapping is required for DNA damage recognition in the Escherichia coli DNA nucleotide excision repair pathway | |
Wang, Hailin ; Lu, Meiling ; Tang, Moon-Shong ; Van Houten, Bennett ; Ross, J. B. Alexander ; Weinfeld, Michael ; Le, X. Chris | |
刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
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2009-08-04 | |
卷号 | 106期号:31页码:12849-12854 |
关键词 | capillary electrophoresis laser induced fluorescence polarization Bacillus caldotenax fluorescence resonance energy transfer |
中文摘要 | Localized DNA melting may provide a general strategy for recognition of the wide array of chemically and structurally diverse DNA lesions repaired by the nucleotide excision repair (NER) pathway. However, it is not clear what causes such DNA melting and how it is driven. Here, we show a DNA wrapping-melting model supported by results from dynamic monitoring of the key DNA-protein and protein-protein interactions involved in the early stages of the Escherichia coli NER process. Using an analytical technique involving capillary electrophoresis coupled with laser-induced fluorescence polarization, which combines a mobility shift assay with conformational analysis, we demonstrate that DNA wrapping around UvrB, mediated by UvrA, is an early event in the damage-recognition process during E. coli NER. DNA wrapping of UvrB was confirmed by Forster resonance energy transfer and fluorescence lifetime measurements. This wrapping did not occur with readily denaturable damaged DNA substrates ("bubble" DNA), suggesting that DNA wrapping of UvrB plays an important role in the induction of DNA melting around the damage site. Analysis of DNA wrapping of mutant UvrB Y96A further suggests that a cooperative interaction between DNA wrapping of UvrA(2)B and contact of the beta-hairpin of UvrB with the bulky damage moiety may be involved in the local DNA melting at the damage site. |
WOS记录号 | WOS:000268667600053 |
内容类型 | 期刊论文 |
源URL | [http://ir.rcees.ac.cn/handle/311016/21487] ![]() |
专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
推荐引用方式 GB/T 7714 | Wang, Hailin,Lu, Meiling,Tang, Moon-Shong,et al. DNA wrapping is required for DNA damage recognition in the Escherichia coli DNA nucleotide excision repair pathway[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2009,106(31):12849-12854. |
APA | Wang, Hailin.,Lu, Meiling.,Tang, Moon-Shong.,Van Houten, Bennett.,Ross, J. B. Alexander.,...&Le, X. Chris.(2009).DNA wrapping is required for DNA damage recognition in the Escherichia coli DNA nucleotide excision repair pathway.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,106(31),12849-12854. |
MLA | Wang, Hailin,et al."DNA wrapping is required for DNA damage recognition in the Escherichia coli DNA nucleotide excision repair pathway".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 106.31(2009):12849-12854. |
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