Study of rat neuronal genes with ordered differential display method

	Study of rat neuronal genes with ordered differential display method
	Kang, JS; Wang, ZP; Jin, ML; Guo, N; Wang, T; Du, YC; Li, RX
刊名	SCIENCE IN CHINA SERIES C-LIFE SCIENCES
	2001
卷号	44 期号:6 页码:601-609
关键词	ODD rat brain EST antisense oligodeoxynucleotides ethology
通讯作者	Li, RX (reprint author), Chinese Acad Sci, Inst Biol Sci, Shanghai Res Ctr Biotechnol, Shanghai 200233, Peoples R China.,
英文摘要	Ordered differential display (ODD) was developed recently and has been applied to systematic comparison of expression profiles of genes. It was further improved with the specific complexing property between biotin and streptavidin by the authors. First, random primer and biotinylated oligo (dT) primer were used to make pools of double strand cDNA. Second, streptavidin-coated PCR tube is used to absorb 3'ESTs specifically to avoid the negative effect of other DNA fragments. In the case of 3'ESTs comparison patterns between embryonic brain and body of SD rat, more than forty differentially expressed genes were cloned and identified. The function of rZIC gene, one of the genes identified and cloned, was studied through ethological experiments. The result showed that rZIC gene was associated with locomotion activity of adult mice.
学科主题	Life Sciences & Biomedicine - Other Topics
类目[WOS]	Biology
关键词[WOS]	EXPRESSION PROFILES ; MOLECULAR-CLONING ; MESSENGER-RNA ; CDNA ; PROTEIN ; SEQUENCE ; CELLS ; DNA
收录类别	SCI
语种	英语
WOS记录号	WOS:000175029600006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2680]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Kang, JS,Wang, ZP,Jin, ML,et al. Study of rat neuronal genes with ordered differential display method[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2001,44(6):601-609.
APA	Kang, JS.,Wang, ZP.,Jin, ML.,Guo, N.,Wang, T.,...&Li, RX.(2001).Study of rat neuronal genes with ordered differential display method.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,44(6),601-609.
MLA	Kang, JS,et al."Study of rat neuronal genes with ordered differential display method".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 44.6(2001):601-609.