Human xenoreactivity is reduced in mice bearing porcine antisense alpha(1,3) galactosyltransferase cDNA

	Human xenoreactivity is reduced in mice bearing porcine antisense alpha(1,3) galactosyltransferase cDNA
	Ma, YH; Zhou, XG; Hu, JH; Fei, J; Xia, GH; Guo, LH
刊名	ACTA PHARMACOLOGICA SINICA
	2001
卷号	22 期号:3 页码:231-238
关键词	epitopes galactosyltransferases heterologous transplantation fluorescent antibody technique antisense DNA reverse polymerase chain reaction Southern blotting swine
通讯作者	Guo, LH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,
英文摘要	AIM: To explore the effect of antisense alpha (1,3) galactosyltransferase alpha (1,3) GT cDNA on production of Gal a (1,3) Gal(Gal epitope) xenoantigen in vivo. METHODS: Transgenic mice bearing the porcine antisense all, 3) GT cDNA (nt 1 alpha - 640) were generated by pronuclei microinjection method. The integration of transgene was identified by PCR and Southern-blot analysis. The expression of murine a(1,3) GT was characterized by RT-PCR. Morphology of the spleen was examined by histological technique. Gal epitope was detected by immunofluorescent analysis. Binding of human natural xenoantibodies (IgM and IgG) and complement (C3c) to cells from mice was determined by flow cytometric assay. RESULTS: Transgenic mice bearing the porcine antisense a(1,3) GT cDNA were born healthy and developed normally. However, necrosis occurred in the spleen of some mice heterozygous for transgene. Cell surface Gal epitope in transgenic heterozygotes was evidently reduced. Substantially less (30 % - 60 %) xenoantibodies in human serum bound to cells from a variety of tissues of transgenic heterozygotes compared with wild-type controls. Consequentially, human complement activation on cells from these mice was reduced by 40 % - 50 %. CONCLUSION: Human xenoreactivity could be effectively reduced by inhibiting the expression of a (1,3) galactosyltransferase with an antisense gene.
学科主题	Chemistry; Pharmacology & Pharmacy
类目[WOS]	Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
关键词[WOS]	DECAY-ACCELERATING FACTOR ; TRANSGENIC MICE ; EXPRESSION ; ANTIBODIES ; CELLS ; PIG ; ALPHA-1,3-GALACTOSYLTRANSFERASE ; EPITOPES ; GENE ; XENOGRAFTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000167403800006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2633]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Ma, YH,Zhou, XG,Hu, JH,et al. Human xenoreactivity is reduced in mice bearing porcine antisense alpha(1,3) galactosyltransferase cDNA[J]. ACTA PHARMACOLOGICA SINICA,2001,22(3):231-238.
APA	Ma, YH,Zhou, XG,Hu, JH,Fei, J,Xia, GH,&Guo, LH.(2001).Human xenoreactivity is reduced in mice bearing porcine antisense alpha(1,3) galactosyltransferase cDNA.ACTA PHARMACOLOGICA SINICA,22(3),231-238.
MLA	Ma, YH,et al."Human xenoreactivity is reduced in mice bearing porcine antisense alpha(1,3) galactosyltransferase cDNA".ACTA PHARMACOLOGICA SINICA 22.3(2001):231-238.