Comparative studies of three type II ribosome-inactivating proteins from the seeds of three species of the genus Cinnamomum

	Comparative studies of three type II ribosome-inactivating proteins from the seeds of three species of the genus Cinnamomum
	Hou, FJ; Wang, BZ; Liu, WY
刊名	PROTEIN AND PEPTIDE LETTERS
	2001
卷号	8 期号:3 页码:193-200
通讯作者	,
英文摘要	Bodinierin, one of the major proteins in the kernels of camphor tree (Cinnamomum Bodinieri), has been identified as a novel type II ribosome-inactivating protein. Using sepharose-4B column chromatography followed by acid precipitation and ammonium sulfate precipitation, bodinierin has been purified to be homogeneous as characterized by SDS-PAGE, Bodinierin was composed of two chains (A-and B-chain) with the molecular weight of 31 and 34 kDa respectively. The reduced bodinierin showed strong inhibitory activity to protein synthesis in the rabbit reticulocyte lysate and the RNA N-glycosidase activity. The bodinierin also displayed the carbohydrate binding activity to agglutinate rabbit erythrocyte. A comparison of bodinierin with cinnamomin and porrectin that were isolated from the kernels of other species of the same genus (Cinnamomum) demonstrated that they had similar structure and biological activities, which provided phylogenetic evidence to the three species.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	EUKARYOTIC RIBOSOMES ; CAMPHORA ; PLANTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000169139300005
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2624]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Hou, FJ,Wang, BZ,Liu, WY. Comparative studies of three type II ribosome-inactivating proteins from the seeds of three species of the genus Cinnamomum[J]. PROTEIN AND PEPTIDE LETTERS,2001,8(3):193-200.
APA	Hou, FJ,Wang, BZ,&Liu, WY.(2001).Comparative studies of three type II ribosome-inactivating proteins from the seeds of three species of the genus Cinnamomum.PROTEIN AND PEPTIDE LETTERS,8(3),193-200.
MLA	Hou, FJ,et al."Comparative studies of three type II ribosome-inactivating proteins from the seeds of three species of the genus Cinnamomum".PROTEIN AND PEPTIDE LETTERS 8.3(2001):193-200.