cAMP elevators inhibit LPS-induced IL-12 p40 expression by interfering with phosphorylation of p38 MAPK in Murine Peritoneal Macrophages

	cAMP elevators inhibit LPS-induced IL-12 p40 expression by interfering with phosphorylation of p38 MAPK in Murine Peritoneal Macrophages
	Feng, WG; Wang, YB; Zhang, JS; Wang, XY; Li, CL; Chang, ZL
刊名	CELL RESEARCH
	2002
卷号	12 期号:1 页码:331-337
关键词	IL-12 cAMP LPS NF-kappa B p38 MAPK
通讯作者	Chang, ZL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Lab Immune Signaling Transduct, Shanghai 200031, Peoples R China.,
英文摘要	cAMP mediated signaling may play a suppressive role in immune response. We previously found that the cAMP-elevators (CTx and 8-Br-cAMP) inhibited IL-12, IL-1a, IL-6 gene expression, but increased the transcriptional levels of IL-10 and IL-1Ra, in LPS-treated murine peritoneal macrophages. The present study examined a possible molecular mechanism involved in cAMP elevators-induced inhibition of IL-12 p40 expression in response to LPS. Our data demonstrated that cAMP elevators downregulated IL-12 p40 mRNA expression and IL-12 p70 production in murine peritoneal macrophages. Subsequent studies revealed that cAMP-elevators blocked phosphorylation of p38 MAPK, but did not affect the activity of NF-kappaB binding to IL-12 promoter (-136/-112). This is the first report that cAMP elevators inhibit LPS-induced IL-12 production by a mechanism that is associated, at least in part, with p38-dependent inhibition by cAMP signaling pathways.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	ACTIVATED PROTEIN-KINASE ; VASOACTIVE-INTESTINAL-PEPTIDE ; INDUCED GENE-EXPRESSION ; ALVEOLAR MACROPHAGES ; HUMAN MONOCYTES ; TNF-ALPHA ; KAPPA-B ; INDUCTION ; INFLAMMATION ; IL-1-BETA
收录类别	SCI
语种	英语
WOS记录号	WOS:000180476800003
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2561]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Feng, WG,Wang, YB,Zhang, JS,et al. cAMP elevators inhibit LPS-induced IL-12 p40 expression by interfering with phosphorylation of p38 MAPK in Murine Peritoneal Macrophages[J]. CELL RESEARCH,2002,12(1):331-337.
APA	Feng, WG,Wang, YB,Zhang, JS,Wang, XY,Li, CL,&Chang, ZL.(2002).cAMP elevators inhibit LPS-induced IL-12 p40 expression by interfering with phosphorylation of p38 MAPK in Murine Peritoneal Macrophages.CELL RESEARCH,12(1),331-337.
MLA	Feng, WG,et al."cAMP elevators inhibit LPS-induced IL-12 p40 expression by interfering with phosphorylation of p38 MAPK in Murine Peritoneal Macrophages".CELL RESEARCH 12.1(2002):331-337.