Identification and characterization of a novel U14 small nucleolar RNA gene cluster in Oryza sativa

	Identification and characterization of a novel U14 small nucleolar RNA gene cluster in Oryza sativa
	Jiang, G; Chen, XL; Li, W; Jin, YX; Wang, DB
刊名	GENE
	2002
卷号	294 期号:1 页码:187-196
关键词	ribosomal RNA methylation homology rice yeast
通讯作者	Jin, YX (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	Small nucleolar RNAs (snoRNAs) are required for ribose 2'-O-methylation of eukaryotic ribosomal RNA. Through computer search in international rice genome database, a novel U14 snoRNA gene cluster, consisting of two U14 snoRNA gene candidates, was found on rice chromosome II. They both have box C/D sequences and a 14 nucleotides (nt)-long complementarity to rice 18S ribosomal RNA (rRNA). Functional analysis of this gene cluster indicated that both were transcribed in vivo and might guide the methylations of C418 in rice 18S rRNA. By using primer extension, 5' and 3' rapid amplification of cDNA ends, the 5' and 3' ends of two snoRNAs were determined. The 52 nt long intergenic spacer of the gene cluster is rich in uridine. The absence of a conserved promoter element in this spacer, the proximity of the genes and the detection of transcripts containing linked U14 snoRNAs by reverse transcript polymerase chain reaction suggest that the rice U14 snoRNAs encoded in the cluster are transcribed as a polycistron under an upstream promoter, and individual U14 snoRNAs are released after processing of the precursor RNAs. (C) 2002 Elsevier Science B.V. All rights reserved.
学科主题	Genetics & Heredity
类目[WOS]	Genetics & Heredity
关键词[WOS]	RIBOSOMAL-RNA ; SACCHAROMYCES-CEREVISIAE ; ARABIDOPSIS-THALIANA ; BOX-C/D ; ELEMENTS ; SNORNA ; METHYLATION ; TRANSCRIPTS ; SEQUENCE ; MOUSE
收录类别	SCI
语种	英语
WOS记录号	WOS:000178855300019
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2552]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Jiang, G,Chen, XL,Li, W,et al. Identification and characterization of a novel U14 small nucleolar RNA gene cluster in Oryza sativa[J]. GENE,2002,294(1):187-196.
APA	Jiang, G,Chen, XL,Li, W,Jin, YX,&Wang, DB.(2002).Identification and characterization of a novel U14 small nucleolar RNA gene cluster in Oryza sativa.GENE,294(1),187-196.
MLA	Jiang, G,et al."Identification and characterization of a novel U14 small nucleolar RNA gene cluster in Oryza sativa".GENE 294.1(2002):187-196.